tailieunhanh - Báo cáo khoa học: Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164

The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensisstrain VTTE-82164 were analysed usingNMR spectroscopy, MS and chemical methods. The LPS contains two major struc-tural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. | Eur. J. Biochem. 270 3036-3046 2003 FEBS 2003 doi Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164 Evaeny Vinogradov1 Bent O. Petersen2 Irina Sadovskava3 Said Jabbouri3 Jens 0. Duus2 . . and Ilkka M. Helander4 1 Institute for Biological Sciences National Research Council Ottawa ON Canada department of Chemistry Carlsberg Laboratory Copenhagen Denmark daboratoire de Recherche sur les Biomateriaux et Biotechnologies Universite de Littoral-Cote d Opale Bassin Napoleon BP 120 Boulogne-sur-mer France department of Applied Chemistry and Microbiology Division of Microbiology University of Helsinki Finland The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide LPS from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy MS and chemical methods. The LPS contains two major structural variants differing by a decasaccharide fragment and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides including the previously found Ara4NP residue in lipid A arranged in a well-defined non-repetitive structure We presume that the shorter variant with R1 H represents the core-lipid A part of the LPS and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain VTT E-79100T showed that it contains a very similar core but with one different glycosidic linkage. Keywords core lipid A lipopolysaccharide Pectinatus frisingensis. P-Ara4N- 1 -P-6 -a-GlcN- 1 -4 -a-Kdo- 2-4 - a-Glc- l-2 -a-Glc- l-3 -a-Gal- l-2 -a-Man- l-4 -a-Man- l-5 -a-Kdo- 2-6 -p-GlcN- l-6 .

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