tailieunhanh - Báo cáo khoa học: Existence of novel b-1,2 linkage-containing side chain in the mannan of Candida lusitaniae, antigenically related to Candida albicans serotype A

The antigenicity ofCandida lusitaniaecells was found to be thesameasthatof Candida albicansserotype A cells, . both cell wall mannans react with factors 1, 4, 5, and 6 sera of Candida Check. However, the structure of the mannan of C. lusitaniaewas significantly different from that of C. albicansserotype A, and we found novelb-1,2 linkages among the side-chainoligosaccharides,Manb1fi2Manb1fi 2Mana1fi2Mana1fi2Man (LM5), and Manb1fi2Man-b1fi2Manb1fi2Mana1fi2Mana1fi2Man (LM6). | Eur. J. Biochem. 270 2565-2575 2003 FEBS 2003 doi Existence of novel p-1 2 linkage-containing side chain in the mannan of Candida lusitaniae antigenically related to Candida albicans serotype A Nobuyuki Shibata1 Hidemitsu Kobayashi2 Yoshio Okawa1 and Shigeo Suzuki3 1 Second Department of Hygienic Chemistry Tohoku Pharmaceutical University Sendai Miyagi Japan department of Nutrition Faculty of Home Economics Kyushu Women s University Kitakyushu Fukuoka Japan 3Sendai Research Institute for Mycology Sendai Miyagi Japan The antigenicity of Candida lusitaniae cells was found to be the same as that of Candida albicans serotype A cells . both cell wall mannans react with factors 1 4 5 and 6 sera of Candida Check. However the structure of the mannan of C. lusitaniae was significantly different from that of C. albicans serotype A and we found novel b-1 2 linkages among the side-chain oligosaccharides Manp1fi2Manp1fi 2Mana1fi2Mana1fi2Man LM5 and Manb1fi2Man-P1fi2Manp1fi2Mana1fi2Mana1fi2Man Lm6 . The assignment of these oligosaccharides suggests that the mannoheptaose containing three b-1 2 linkages obtained from the mannan of C. albicans in a preceding study consisted of isomers. The molar ratio of the side chains of C. lusitaniae mannan was determined from the complete assignment of its H-1 and H-2 signals and these signal dimensions. More than 80 of the oligomannosyl side chains contained b-1 2-linked mannose units no a-1 3 linkages or a-1 6-linked branching points were found in the side chains. An enzyme-linked immunosorbent inhibition assay using oligosaccharides indicated that LM5 behaves as factor 6 which is the serotype A-specific epitope of C. albicans. Unexpectedly however LM6 did not act as factor 6. Keywords Candida lusitaniae mannan NMR analysis serotype A b-1 2-linkage. The first description of the serological heterogeneity in Candida albicans was reported by Hasenclever and Mitchell 1 . These researchers revealed that this .