tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 92

Introduction to Modern Liquid Chromatography, Third Edition part 92. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 866 TROUBLESHOOTING Table Leaks Section Symptoms and Sources Location Sub-location Symptom Possible Source Solution Pre-pump Fitting Loose fitting Tighten or replace Proportioning Loose ordamaged Tighten valve or replace manifold low-pressure mixing valve manifold Pump hittings Loose or damaged Tighten or replace Checkvalve Loose Cross-threaded 1p Seal damaged Tighten m Replace may require new pump head Replace Pumpseal Worn seal Rephue Auxiliary component Loose fitting damaged component Tighten or replace High-pressure Stainless steel Loose or Telten l 4-turn clean or fitting contaminated rePlace PEEK 4 contaminated Turn off pump reneat tcle n eTneTc Autosampleroe manual injector Fittings p geedp e1 See fittings edpustrnents Sections Low-pressure needle seal Worn or looseeeal Adjuntorreplice Highipressureneedle seal geedp e1 seal AdpusSnr repluce Cross-port leakage Damaged rotor seal Clean replace rotor seal may require stator s 1 replacement. External leaksnotat fittings Worn rotor seal knepffident _ rotor-to-seal pressure CPan ieplacnrotoesiul Adjust j Seal-pack failure Waters onlyl WeirnOT dameged seals Service or replace TROUBLESHOOTING TABLES 867 Table Continued Location Sub-location Sympton Possible Source Solution Column Tube connections Loose or damaged See fittings adjustments Section End-fitting Loooeor damaged TLgiten may require srw column End-fitting on Loose or damaged Tighten replace seal may cartridge column fitting damaged seal rvnktrenewasiernbln Detector Connecting fittings Loose or damaged Reefittaigsadlustrnevts . _ Sections Cell leaks Loose fitting Tomtit Over-pressure Rsmnkeeourceoeexcrtsivs damage pressure Fniled windswseal Tsljvstoe replace Non-UV detector See Chapter 4 and operator s manuals e atessete Table Pressure Problems Symptoms and Sources Symptom Location Symptom Possible Source Solution