tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 90

Introduction to Modern Liquid Chromatography, Third Edition part 90. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 846 TROUBLESHOOTING pulsations Section or leaks Section . In the case of Figure isolation of the HPLC system from the electrical circuit as well as pump and detector troubleshooting could not solve the problem. Rather movement of the system away from its original location reduced the frequency of the noise and with sufficient distance eliminated it. Although the source of noise was never definitively identified the noise wasattributed tomterference froman electromcairfilter 21 . Artifact or ohosd peakn z dè iTom gidd dwts aepresaot st special type of long-term fOiscs dhiww OiFipure afrodient od 5-80 ACN-phosphato buffer pH-d inl5rmndtei Witha 1olf on 8i B 7 . Ideally thi traft Sdirt shouidbr frteoa a1 s Oi-SOsbh itnt gradifno -thr batotine drift is cau1rdbn gffdrancosinapforbonc1 oSddeA- and B-eolvenit as d-sceesed above . The mostlikely sourceof peaksin blank gradients ilfondemhdotion nedhu A-solvent fOtcr 1Pesfcnntaminantseeoddo1oncen1rate at thohedlrfeCs do-uma during equilibrationbetweenemr Saddwed yeheir elutiooddringthe uriotmi. A simple wayda eonfomA-to ven1chnfâhou1tion s edancroasetheeqiaiaeutiobtime between rhns flowihgA-solhOdd-. Of dre oonsrminantd oritefrnm bii A-r l of t. ah the peaks ahoidd inereescrouailly iopsdpdrWdnro bis mcreasedeqiiilibsofidn firm . a larnervoiumebfA11divepf WiPlanmrroaseoScbSleotedcnntaminanls . In the preseniaxoaidta e O-mh ute rbuiiibratibn oSHghoe lh1onareta 30 minuter Pig. K s i tleA nrnOsoofwas repeatedo It tan beserutha1dhth teaS s are each abogtdbrearime- arger socobtemmatronbf AeA-tdlvend ecpn rnnd. Further isdhtidn nfioa hrobeemidoatifieO be oH-meter oreOeasthelouace of contamination hr ihideocmplr d . comparesrestdtsfor a hlsoO uao made withbufferpreoauePbd tipping tbopn probs a the buhtared cdjces tSepH Fig. stOht results irece tha isedtbuffeo made w thout cpaoast whOi fin pH probe . A1ilitiopW enamplrt o o. ueoou obofd