tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 87

Introduction to Modern Liquid Chromatography, Third Edition part 87. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 816 TROUBLESHOOTING and outlet check valves seldom are interchangeable and if they are not clearly marked an identifying mark should be scribed on the check-valve body to indicate position and flow direction. Some check valves will come apart when inverted so it is wise to check for this prior to sonication or you may be surprised to find small parts in the sonicator after a cleaning attempt. We recommend placing each check valve in a separatebeakerwithenoughmethanolorisopropanol tocoverthe check valve this wthfixleaky chcek valves mostu tlie time presumrblybyremoving unwauted crntemsnents item the ball and or- seatof hcUecknaatne nomet neart clrtn ihe parttmaleuhol then careft Siyr asv mP e ihetn uting forcepkaeoidcontactingthe inlcrnoS reive parts with jrapoa elutn orfingses berausen smahhitklfiper 3 11 00 cause the n Udii i l eiscursiononrhe possible causes of endeorrectiovs tor check-valvestiokine. teak iiitection Mobrle-phes .e . moy fe oS pevygg-ginl k Drips leak nebs make ocaiionof . 1 Vgieeriaes atcd eivhKe crysrallrnc may show up on a slowly leaking fitting even where no liquid is obvious. A simple leak detector for hard-to-find leaks can be made from a piece of thermal-printer paper . a credit-card receipt .Cut a narrow pointed strip of thermal paper . 1 x 5 cm ppintsdvtoneendiekduIeiCrpoinIedrnd erurcVctutaectcd httingei seals or other postibk aavee nvi11 iornbloek whev e anntaclc organic solvent pis vckke usnfhlfoslncatingleaks sege arshgrkIo dvrcct byetPvu means. R ep airmg CiIImgCeakp Correcting a kaMneki mao be an WvusIv vngPienmg rhe feting oa np wen to see if a leak can be stopped. If this does not fix the problem disassemble the fitting rinse it anhreassemble it or replace the ferrule with a new one. Do not overtighten a stainless steel fitting because the internal parts can distort enough that the .

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