tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 84

Introduction to Modern Liquid Chromatography, Third Edition part 84. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 786 SAMPLE PREPARATION Figure Isolation of albuterol from human plasmabymeans of solid-phase extraction SPE . columns that incorporate sample-preparation and unlike SPE cartridges are plafenumbrrs and mM otasdect1 l for the analysis oflo v-biolnculiir-wciglitdbugr inciribipuribcb aiidmclssbolitcs 50-52 Many variations of these packings have been described 1 internal-surface reversed-phases 2 shielded hydrophobic phases 3 semi-permeable surfaces 4 dual-zone pSirseS anb 5 misedfun tfonal 50 for adescriation rnd tabulationofi i mmerdarpro ustb Dual-mods bneoudpackings arc usedin the mostpopuiar RAM coltmcib These pacings are usenrypicallyfor -hercrlRtisoSdrobslnSfood erauseproSems present in shrsc campics wiU accumuCadecnsRPC sofomn lecdingsoiss failure SOLID-PHASE EXTRACTION SPE 787 after a few injections. The packing consists of small-pore particles with a Cg or Cig layer covering the inside of the pores and a nonretentive hydrophilic layer covering the exterior of the particle. Proteins are unable to access the pores because of their large size and are unretained by the particle exterior conscquently they pass through the column with k s 0. Small-molecule analytes can enter the pores and are retained sufficientlytoeluteafterthe proteins oftenusinggradient elution . Becauseproteins are not retoinednn theue eoltetnns ncelumncange osengora consideranienumOee of iemecooemust betoeerioeO inohe choiceormoOiie-phasapH and organic uolveot gthcewise erolem yredoitctigncan ocrur wishfoulingoe the column. AlterHetively a RAM celumocan etironttee gia arwildiingvalve tea conventiooal RPCcotgmn gvosdimrnsienal tepaeptigdlagSomn-rmitcOinOiSrctiyh . Thrvalaeis lnitiallooosi-ienedfoenlutSonoS fheRRMceinmo -e waste-and the samnlriimldcted aftertOe arnsdn-leavtthecglumH the neine is rwischeH to cgnnecf-he ewo columns. Analytes are then eluted from the RAM column and enter the RPC coiumnforfurther proce-tinei .

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