tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 81

Introduction to Modern Liquid Chromatography, Third Edition part 81. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | CHAPTER SIXTEEN SAMPLE PREPARATION with Ronald Majors INTRODUCTION 758 TYPES OF SAMPLES 759 PRELIMINARY PROCESSING OF SOLID AND SEMI-SOLID SAMPLES 760 760 Sample Drying 762 k n 763 SAMPLEPREUNRATIONPORniEFPDSAMPLES P64 LIQUID-LKiUID EXTREUCTtON 764 ThUOry 766 Practk. 76E Problems 768 Apjnoadns loLi qui d-Liquid LutAFtioii 77a S03ID-PLASEEXTRAUSinN PTR SPUarnl HPLCCompared 772 Us oESPE 772 SPUDevices 7TE SPUApparatus 777 R7S Examok oiSPEMethod Dcvelapment Isolation of Albuterol from HumaoSlaima 784 Spa tiit yopiss FtSEE 785 MEMBRANETECHNIQUESINSAMPLE PREPARATION 790 16 8 SAMPLE PREPARATON METMCd FOR COEID SAMPLES 791 TraditionaHEetraceiEn MetSods 792 MaOetnMathods foaEnsaarirng SoEds 793 C03UMN-SWlTPTPNG 7RD Introduction tuModerzEiccidPhremaiosreplupTDird Edition by Lloyd R. Snyder Joseph J. Kirkland and John W. Dolan Copyright 2010 John Wiley Sons Inc. 757 758 SAMPLE PREPARATION PREPARATION FOR BIOCHROMATOGRAPHY 797 SAMPLE PREPARATION FOR LC-MS 800 DERIVATIZATION IN HPLC 802 INTRODUCTION Sample preparation is an essential part of HPLC analysis intended to provide a representative reproducible and homogenous solution that is suitable for injection into the column. The aim of sample preparation is to provide a sample aliquot that 1 is relatively free of interferences 2 will not damage the column and 3 is compatible with the intended HPLC separation and detection methods. The sample solvent should dissolvent the mobile phase without affecting sample retention orresolution and withoiettnCecfeeheoteitridi te t tK i . Ilmuyariobenecusearyto concentrate the analytes andtoiderinatire them forimpeoaedOefccteon ci aetterteoaeatien. Sample preptrationbeginnae thetoint ofcohecsiov extinPato svmpfesnjectfon onto the HPnCcolnmn end encpmoaoees