tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 76

Introduction to Modern Liquid Chromatography, Third Edition part 76. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 706 ENANTIOMER SEPARATIONS Chiral Crown-Ether CSPs Stereoselective CSP-analyte complexation with chiral crown-ether CSPs and their first application as CSPs were pioneered by Cram and coworkers 127 . In this early work two 1 1 -binaphthyl units were incorporated into a crown ether as replacement elements of ewoethylene gt Py i y U-kno n - Structural anelogues of suchld -binaChthyl-dcrivcd chiral crown-ether based CSPs werelaterdevelopedby Shinbo sgroupusing 3 3hdiphenyl-l r-binaphthyl -or fi -diot tylsUpl -i iioghiskdbybiw ithiUy rha i -i rov nd Sonamically coated onto octadecyl-as -i a 12a rrelatad CSPhasbeen commercielizel asCrnwnsakCR by Daicel eudCWecl TeeOncltgies i3ig. . Smeethry aae o3synthrtic origin both bn- ni letsa s fiens s wiiy bppasine elutionordbrs areavailabie detcbed as CrowntbP 3R and CR - i Urnioicuil asaioguet cseavaiirUle b-nel ur- 18-crown-6 6e65iU ei tet i albarUo -flib arid . lartaric e i3- coi b J mteS into crown ethers-hal ir bivalrntly inmo dit d vis twvcprOoov-ie eunatisnalitiet onto 3-ambloprpvylsCad-t0ias arecnmmerciallc a Vi iibbb-al Cbh roSil RCA urd SCA - from Reois MortonGrove J-cndChirn-Hscc-CRil from K-MAd K3ceui . The appl rt-oct oV such ch-ral uroum-ether-based CSSt are asr-miaUly restricted otrann aminns comcsivingmciolgamino ecidi immoiuld estecs amino alcghois isO chieaiUrugvwilh Sreeprimarpamino fpictionalisy. Tspicslly aqueous mobiledHa44S wi-h dH betwreo 1 srtd 3k ire regmredtoentdaesull protonationoO Ki3 arrsStlng ehiral ammonium ions can bind to the macrocyclic crrwe s. incsuricn rmnpli Kin a nsd-i i t lv at1Us hydtogen-sond formation between riie ammoniom rincud rC s OsrceoioecKS o- dmcc wn Fig. Cnnp p a i -ty mas bepsvtrned t ii searic dactorsariting from ibs substituemssif Pte d uae ammomrmloui andthereaidiie- vstached tothachlrai moieties tUalareinlorpyraPed mis ehe 18 2V sc-dic conditionsspprar- plso irnpomm yp suppress sHand my .

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