tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 67

Introduction to Modern Liquid Chromatography, Third Edition part 67. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 616 BIOCHEMICAL AND SYNTHETIC POLYMER SEPARATIONS anion-exchange conditions the negative charge of a single phosphate group is insufficient to counteract the electrostatic repulsion of N-terminal and side-chain amines and monophosphorylated peptides elute in or near the void volume. When a weak anion exchange column is operated in the ERLIC mode with higher -ACN hydrophilic interaction drives an increased interaction of phosphates enabling the retention and resolutionof mono-and multiple-phosphorylated peptides. ERLIC has also been applieU sotha enrieiamout aud separationofsialylated f ycopeptiOos 93 . MuOekHmeraouiM UqCP Chramirtogi-nphy MpLC m Prrteomt Expressiou nreieomicsseekt eo nhasicoeriec the emirs oomplemect of ors lei s expressed in oceli oeeiftueunelerdePneL conditions ts C tlIfe yTl te iCentieomg proteins that are PiOOcfetisiatey expreetuU undeotnosfoonditions. OucOpeotciiLase candidareeforbrnmarkers r beuserl t ia rga dm mrOTr i as sol . or as targetsfor thcrapr bottom-up proteomics the entibe protein conlensofaceC artissuu lysateis aleuuof wisfia pr Seoiytic 60 1 1101 resultant oectidetare sepshatt d bochromatography andchatocterieedOyeohdem mass specfromeny hC-MS MS .Thh cEalieEgefornottomIopproteomicnittCe enormous oomploxiinof the popero hnmanprooeomeitestimetod to contain from 00 ee0IoLmillienoPoteint ofwhlenl 000eoLP 000areexpresied at any giren time. Lroteolotio digeetioo . will o anean yieldo cporoxtmotaiy2P peptides fes pachpcotctn io fSe digeotsebtamun Pome Opsute can brrnpaeleO to contain 20P 000so5d0 fbofureoe tPs proseomict experimenté tPalvirepailo overy aumcondm pf itip sameSe Pen anaiySo so ihat an extraordmary neoolvengpower is ofuUecomponentsofsneO a complexmmuoe o beyond tOe cauabiliy oO a tinylo ehrcmlltogroplOcmode. Proteomics siu-Hes acquire 6 0 atom moremodeepf chromatnoroohy multidimtLtionalliwniWchromanogsapOn MDLCiwith MS Peleptido