tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 53

Introduction to Modern Liquid Chromatography, Third Edition part 53. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 476 COMPUTER-ASSISTED METHOD DEVELOPMENT emphasize commercial computer-simulation software that can predict separation as a function of one or more experimental conditions by means of experimental data from a few preliminary separations. Because of this software s ability to predict separation as opposed to carrying out real experiments we can reduce the amount of experimental work that is required while ensuring the best conditions for the finae meahod. A simple example foe isocratic RPCisahepredictionof separationasafunc-tion of modile-mlaase strenath B tom idusappetcationi two experimentaS rees are requiredprtorto compuSariCmplatton. In the exampleaS eeaort iments ustnaamopiieuhase oeiOand ea B tFac eenditiooa linrhenged aee shown in diauea data are entered into the computer retention times for each soles ineach aim B aalues for the Swo eunt piO UOPo B and other rnherPnentalconditfont A- end B-solvenecomuuiltiontiColomndimen-sions and portick raze Fow rateiSemeeratina Tda computer can now Ce ieteaenontad fas p-sCK t fo a of aoparrtion sae i attpo ap o lonca tion of ehangei in mobita-phate B flow rape oohlnuli0mcnsions ondnubaale size. The mpsa eurfulinfoemadon meovPded by computersmmPetiop ismsuall c resolution mop .is U uelni tl ot Hoere Sortlle separatfon of thia mixtere of seven acais anO basei. A resoiutfodmaoit r miotof0U3oripoal resalatlah Rs. for the twofoapdeei oineO pe nr aeufpnctiopofr0e cooditfobaI too t diat were variedm the initial experimental runs Section and Fig. provide additional informatinnon rearlutiop 3PS . Iu Poo apas pl wos ranid am JOS is plotted asafunctionof B. tn Fiacre that moxrniam retoPitfon occurs for aheee Iifteecot conbitions 2 Bo wiU1Phrlargest eetalutiop for 17 .AVect oal yt ii ta sunadoted chroh lt amCm amp vaioeofr tP1 as illustrate in lagosc for 0ie atter optmiiois Boalues. The computtr can provide etlath3r information tor each at ease