tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 24

Introduction to Modern Liquid Chromatography, Third Edition part 24. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 186 DETECTION mass spectrometric MS detector is the most popular hyphenated HPLC detector in use today other hyphenated detectors are discussed in Section . MS detection has become the standard detector system for bioanalytical methods the analysis of pharmaceutic al compounds in biological systems . plasma or urine . MS detectors are also widely used in the R D setting to provide structural information or confirmationofunknowns althoughitdoes nothave the mass-resolution capability of traditional eecnd-alone detectors comeintwo popular smg e-otngcs-ciectoia eomsoinws calledanMSD mitssosloctioe detector is utsd tomeasura ouiiupciornc epeciasfos eaoO analyte. oftentfia protonatec moleeulat ion M H . Within a given run moee ttanona smpsee ion can bemonitoreO bo switphmgbacnAndtosthCrtweeodiffeernt1r cvAlusepr scanning Ostwscnions. tnittinoiepts 01 0 0 owe n- deteotion ate ueaerred io as LC-MS. Amorc-TwoPom detectordesign isolates thoorisnoru mmc snncies pareat or precurins n feagmmtsit into 11 10 bls trr oe 010 and moniuoesoneor moraosthene productions. TPis multiple reanunn M c oOnezo cdR i givns andednclsotivlty idcu ths transition from piwrirsnn to proWum ionos uneS atc sigoannre ofosASch-o systems arereferred too-LC-MU w-Urrecn toLC-MStMO wOsn thltsproiiic technique Is ue aod LCcMSfostPs singie-itagemethodolpuyorwhrn iiisnot important whethen sO-system-s OCA Oh oeLC-MOcMS. a 10x 61 300 80088 of MS detection ns naplisn trgraS eng elatioacan brfnhhil S3- much of wOich-t aquA-lnvo-ldfos ow tio cnaratioe . Additionas informatinn about LCoRO ud CC-MStMS drtectioacaa bntoanU is ds obse ichrl tonhe subject . pe -4 . Ostafac e . . . The developmsnirethr Mc detectar latert-sr epera iips Cismnc imyorteot factor in the succu sl itns lSc on of mass spectrometry as an HPLC detection technique. MS detectors manipulate and detect ions in the gaseous phase so for the