tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 15

Introduction to Modern Liquid Chromatography, Third Edition part 15. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 96 EQUIPMENT of tubing wall in Fig. . With properly designed systems sub-milliliter volumes of tubing can be used for each solvent with degassing effectiveness equivalent to extended vacuum degassing under static conditions for most HPLC operations 5 . Although not quite as effective as helium sparging for most users the convenience of in-line vacuum degassing and the cost of helium have made this the preferred degassing technique in place applications where dissolved-oxygen eoncenssetionr eue criticai . some fluorescence end ghettochemical rutectionmethoaiiorwhcreexttemslc iow aodcoostandconcentrationu of dissolved gmmigh dunqgn-ained seniisivisgredsactidCrigCex detectionjgcontinuons deliumcpar og-gflowed C. in-lmnvacoxm dagoscingis thr best choice Xg. TUBINGANDHTTiNGS Tubing andtht-Uemgsused to connect tubing to various HPLC system components are required for transporting the mobile phase and sample through the chromatograph. If reasonable care is taken in the selection and use of tubing and fittings problems will improper selection and use can generate unwanted extra-column volume Section which can compromise the separation especiallyfor rmefl peakvthimet. mGditihualseugnical inlorueioou on tubing aed figgingr can beteondgncholnseene-ia-h rnhln mmsofetUu cCt literature shehas disca-h-nnrefces tathedimenstons and threans ze-of tobinganOfitsigg-ingdh-ishumtstgtgally Seautlgdai oeducimai inches beedesc Cusitthewac sheyaroeommoIgysupp ien intOeUhitcd toteis These prodnctsare avaihblein cnnth of caution iSdothEnghsh andmettic veesionsdfsimilarprodgcts are used de stem to label themciearly cvcn 8 theo ma-ree-e to dtedgetherm smse casrs si-re damage toehe haatdra-raX may retldr. TulCine Lnw-Psessuredhh nd For pressure1 l s than Mi 0pvi .Cymric tobinggenerally is Mutadle. Th0 two primary aeration of tire mogiie phase