tailieunhanh - Introduction to Modern Liquid Chromatography, Third Edition part 14

Introduction to Modern Liquid Chromatography, Third Edition part 14. High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. . | 86 BASIC CONCEPTS AND THE CONTROL OF SEPARATION 85. . Lin L. R. Snyder and T. A. McKeon J. Chromatogr. A 808 1998 43. 86. C. T. Mant and R. S. Hodges in HPLC of Proteins Peptides and Polynucleotides M. T. W. Hearn ed. VCH New York 1991 p. 277. 87. K. Yanagida H. Ogawa K. Omichi and S. Hase J. Chromatogr. A 800 1998 187. 88. T. Baczek R. Kaliszan H. A. Claessens and M. A. van Strafen LCGC Europe 13 89. V. Spicer K. . Wilkens and O. V. Korkhin 79 2000 87S2. 90. P. C. Sedrk M. J. Kamlee . Tan . Abeaham Anal. Chem. 5h 1985 2r7l. 91. C. F. Poole andS. J. Chromatogr. A 965 2002 263. CHAPTER THREE EQUIPMENT INTRODUCE E RESERVOIRS AND SOLVENT FILTRATION 89 Reservoir Design and Use 90 Mobile-Phase Filtration 91 M0BILE-PHASEDEGAPSING 92 Degassing Requirements 92 Helim Sparing 94 Vacuum and In-line Degassing 95 TUBING AND FITTINGS 96 TubAg 9U Firnngs 99 PUMPINGSYSTEMS 104 Reciprocating-Piston Pumps 104 OH-PgeSGxGp 109 Gradisnt Systems 112 Spnuial Applinstiom 112 AUTOSAMPbERP 113 Six-Post InjectionValves 114 AmosumplesDse Tt sg6 Sanipln-SizcEOfects r d OtliprValviApplicaiioiiS1 122 C0LUMNPVS19 E25 TcmpsratvocsCmtrvlRo irrmcnts s45 Onil esigsii SS6 DATA SYSTEMS 127 Experimental Aids 127 SyEtemPbntrA UP9 DataColkc1 11 S29 DataProceeci ReE9rtGiniiitiub 130 RegulatoryFiditSeoi 130 EXTRA-COLD MNESFECTS 131 Introduction to ModernLiquidChromatography ThirdEdition by Lloyd R. Snyder Joseph I. Kirkland and John W Dolan Copyright 2010 dohnWiled Sons Inc. 87 88 EQUIPMENT MAINTENANCE 131 System-Performance Tests 131 Preventive Maintenance 138 Repairs 143 INTRODUCTION Equipment design for modern HPLC is in a mature state. With certain exceptions . high-pressure applications .