tailieunhanh - Báo cáo khoa học: Poly(ADP-ribose) The most elaborate metabolite of NAD+ Alexander Burkle

One of the most drastic post-translational modification of proteins in eu-karyotic cells is poly(ADP-ribosyl)ation, catalysed by a family enzymes termed poly(ADP-ribose) polymerases (PARPs). In the human genome, 18 different genes have been identified that all encode PARP family members. Poly(ADP-ribose) metabolism plays a role in a wide range of biological structures and processes, including DNA repair and maintenance of genomic stability, transcriptional regulation, centromere function and mito-tic spindle formation, centrosomal function, structure and function of vault particles, telomere dynamics, trafficking of endosomal vesicles, apoptosis and necrosis. . | iFEBS Journal MINIREVIEW Poly ADP-ribose The most elaborate metabolite of NAD Alexander Burkle Department of Biology University of Konstanz Germany Keywords PARP tankyrase poly ADP-ribose DNA damage DNA repair genomic instability centrosome centromere telomeres mitotic spindle Correspondence A. Burkle Department of Biology BoxX911 University of Konstanz D-78457 Konstanz Germany Tel 49 7531 884035 Fax 49 7531 884033 E-mail Website http . One of the most drastic post-translational modification of proteins in eukaryotic cells is poly ADP-ribosyl ation catalysed by a family enzymes termed poly ADP-ribose polymerases PARPs . In the human genome 18 different genes have been identified that all encode PARP family members. Poly ADP-ribose metabolism plays a role in a wide range of biological structures and processes including DNA repair and maintenance of genomic stability transcriptional regulation centromere function and mitotic spindle formation centrosomal function structure and function of vault particles telomere dynamics trafficking of endosomal vesicles apoptosis and necrosis. In this article the most recent advances in this rapidly growing field are summarized. Received 5 May 2005 accepted 14 July 2005 doi Introduction The life cycle of poly ADP-ribose NAD NADH is among the most versatile biomolecules as it can be used not only as a coenzyme for a large number of oxidoreduction reactions but in its oxidized version can also serve as substrate for several different of ADP-ribosyl transfer reactions which are the overarching theme of this minireview series. The covalent transfer onto glutamic acid aspartic acid or lysine residues of target proteins acceptors followed by successive transfer reactions onto the protein-mono ADP-ribosyl adduct and subsequently onto the emerging chain of several covalently linked ADP-ribo-syl residues is the basis of the formation of poly