tailieunhanh - Báo cáo khoa học: Emerging tools for real-time label-free detection of interactions on functional protein microarrays

The availability of extensive genomic information and content has spawned an era of high-throughput screening that is generating large sets of func-tional genomic data. In particular, the need to understand the biochemical wiring within a cell has introduced novel approaches to map the intricate networks of biological interactions arising from the interactions of proteins. | iFEBS Journal MINIREVIEW Emerging tools for real-time label-free detection of interactions on functional protein microarrays Niroshan Ramachandran1 Dale N. Larson2 Peter R. H. Stark2 Eugenie Hainsworth1 2 and Joshua LaBaer1 1 Harvard Institute of Proteomics Department of BiologicalChemistry and Molecular Pharmacology Harvard Medical School Cambridge MA USA 2 Technology Engineering Center Department of BiologicalChemistry and Molecular Pharmacology Harvard Medical School Boston MA USA Keywords carbon nanowires cell-free system colorimetric resonant reflection label-free detection MEMS cantilevers nanohole array sensors protein interactions protein microarrays protein purification selfassembling protein arrays surface plasmon resonance Correspondence J. LaBaer Harvard Institute of Proteomics Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School 320 Charles Street Cambridge MA 02141 USA Fax 617 324 0824 Tel 617 324 0827 E-mail josh@ Received 27 May 2005 revised 16 August 2005 accepted 30 August 2005 The availability of extensive genomic information and content has spawned an era of high-throughput screening that is generating large sets of functional genomic data. In particular the need to understand the biochemical wiring within a cell has introduced novel approaches to map the intricate networks of biological interactions arising from the interactions of proteins. The current technologies for assaying protein interactions - yeast two-hybrid and immunoprecipitation with mass spectrometric detection - have met with considerable success. However the parallel use of these approaches has identified only a small fraction of physiologically relevant interactions among proteins neglecting all nonprotein interactions such as with metabolites lipids DNA and small molecules. This highlights the need for further development of proteome scale technologies that enable the study of protein function. Here we discuss recent advances in .