tailieunhanh - Báo cáo khoa học: Advances in functional protein microarray technology Paul Bertone and Michael Snyder

Numerous innovations in high-throughput protein production and micro-array surface technologies have enabled the development of addressable formats for proteins ordered at high spatial density. Protein array imple-mentations have largely focused on antibody arrays for high-throughput protein profiling. However, it is also possible to construct arrays of full-length, functional proteins from a library of expression clones. | iFEBS Journal MINIREVIEW Advances in functional protein microarray technology Paul Bertone and Michael Snyder Department of Molecular Cellular and DevelopmentalBiology Yale University New Haven CT USA Keywords biochemical interactions high-throughput proteomics large-scale protein production microarray analysis protein arrays Correspondence P. Bertone Department of Molecular Cellular and DevelopmentalBiology Yale University New Haven CT 06520 USA Fax 1 203 432 3597 Tel 1 203 432 6139 E-mail Received 28 June 2005 revised 9 September 2005 accepted 14 September 2005 Numerous innovations in high-throughput protein production and microarray surface technologies have enabled the development of addressable formats for proteins ordered at high spatial density. Protein array implementations have largely focused on antibody arrays for high-throughput protein profiling. However it is also possible to construct arrays of fulllength functional proteins from a library of expression clones. The advent of protein-based microarrays allows the global observation of biochemical activities on an unprecedented scale where hundreds or thousands of proteins can be simultaneously screened for protein-protein protein-nucleic acid and small molecule interactions. This technology holds great potential for basic molecular biology research disease marker identification toxicological reponse profiling and pharmaceutical target screening. doi DNA microarrays have become ubiquitous in genomic research evident by their widespread use in profiling gene expression patterns mapping novel transcripts detecting sequence mutations and deletions and locating transcription factor binding sites. Although microarray experiments are invaluable for large-scale sequence analyses little can be inferred from these studies about the functions of gene products. In contrast to the high-throughput HT experiments afforded by DNA arrays those designed to elucidate

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