tailieunhanh - Báo cáo khoa học: Structures of the O-polysaccharides and classification of Proteus genomospecies 4, 5 and 6 into respective Proteus serogroups

An acidic branched O-polysaccharide was isolated by mild acid degrada-tion of the lipopolysaccharide (LPS) of Proteusgenomospecies 4 and stud-ied by sugar and methylation analyses along with 1 H and 13 C NMR spectroscopy, including 2D COSY, TOCSY, ROESY and H-detected 1 H, 13 C HSQC experiments. | iFEBS Journal Structures of the O-polysaccharides and classification of Proteus genomospecies 4 5 and 6 into respective Proteus serogroups Krystyna Zych1 Andrei V. Perepelov2 Matgorzata Siwinska1 Yuriy A. Knirel2 and Zygmunt Sidorczyk1 1 Department of GeneralMicrobiology Institute of Microbiology and Immunology University of bodz Poland 2 . Zelinsky Institute of Organic Chemistry Russian Academy of Sciences Moscow Russian Federation Keywords Proteus O-antigen O-serogroup serological classification 3-acetamido-3 6-dideoxy- D-glucose Correspondence Z. Sidorczyk Department of General Microbiology Institute of Microbiology and Immunology University of bodz Banacha 12 16 90-237 Lodz Poland Tel 48 42 6354467 Fax 48 42 6655818 E-mail zsidor@ Received 16 May 2005 revised 30 August 2005 accepted 5 September 2005 doi An acidic branched O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide LPS of Proteus genomospecies 4 and studied by sugar and methylation analyses along with 1H and 13C NMR spectroscopy including 2D COSY TOCSY ROESY and H-detected 1H 13C HSQC experiments. The following structure of the pentasaccharide repeating unit of the O-polysaccharide was established which is unique among Proteus polysaccharide structures a-D-Glcp 1 ị 2 4 -0-D-Quip3NAc- 1 6 -p-D-GlcpNAc- 1 4 -ị3-D-Galp A- 1 3 -a-D-GaỊpNAc- 1 - where Qui3NAc stands for 3-acetamido-3 6-dideoxyglucose. Based on the O-polysaccharide structure and serological data we propose classifying Proteus genomospecies 4 into a new separate Proteus serogroup O56. A weak cross-reactivity of Proteus genomospecies 4 antiserum with LPS of Providencia stuartii O18 and Proteus vulgaris OX2 was observed and is discussed in view of a similarity of the O-polysaccharide structures. Structural and serological investigations showed that Proteus genomospecies 5 and 6 should be classified into the existing Proteus serogroups O8 and O69 respectively. .