tailieunhanh - Báo cáo khoa học: Functional interaction between RNA helicase II⁄Gua and ribosomal protein L4

RNA helicase II⁄Guais a multifunctional nucleolar protein involved in ribosomal RNA processing inXenopus laevisoocytes and mammalian cells. Downregulation of Guausing small interfering RNA (siRNA) in HeLa cells resulted in 80% inhibition of both 18S and 28S rRNA production. The mechanisms underlying this effect remain unclear. Here we show that in mammalian cells, Guaphysically interacts with ribosomal protein L4 (RPL4), a component of 60S ribosome large subunit. | iFEBS Journal Functional interaction between RNA helicase II Gua and ribosomal protein L4 Hushan Yang Dale Henning and Benigno C. Valdez Department of Pharmacology Baylor College of Medicine Houston Texas USA Keywords ribosomalprotein ribosomalRNA biogenesis RNA helicase nucleolus Correspondence B. C. Valdez Department of Pharmacology Baylor College of Medicine Houston TX 77030 USA Fax 1 713 798 3145 Tel 1 713 798 7908 E-mail bvaldez@ Received 11 April 05 revised 19 May 05 accepted 9 June 05 doi RNA helicase II Gua is a multifunctional nucleolar protein involved in ribosomal RNA processing in Xenopus laevis oocytes and mammalian cells. Downregulation of Gua using small interfering RNA siRNA in HeLa cells resulted in 80 inhibition of both 18S and 28S rRNA production. The mechanisms underlying this effect remain unclear. Here we show that in mammalian cells Gua physically interacts with ribosomal protein L4 RPL4 a component of 60S ribosome large subunit. The ATPase activity of Gua is important for this interaction and is also necessary for the function of Gua in the production of both 18S and 28S rRNAs. Knocking down RPL4 expression using siRNA in mouse LAP3 cells inhibits the production of 47 45S 32S 28S and 18S rRNAs. This inhibition is reversed by exogenous expression of wild-type human RPL4 protein but not the mutant form lacking Gua-interacting motif. These observations have suggested that the function of Gua in rRNA processing is at least partially dependent on its ability to interact with RPL4. Ribosome biogenesis is a complicated cellular process which occurs in the nucleolus 1 . The entire scenario begins at the end of mitosis and includes ribosomal DNA transcription pre-ribosomal RNA pre-rRNA modifications and processing as well as assembly of rRNAs and ribosomal proteins into preribosome subunits which are then exported to the cytoplasm to form the mature ribosomes 2 . Errors in this process are reported to be .

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