tailieunhanh - Báo cáo khoa học: Myocyte enhancer factor 2 (MEF2) is a key modulator of the expression of the prothoracicotropic hormone gene in the silkworm, Bombyx mori

Prothoracicotropic hormone (PTTH) plays a central role in controlling molting, metamorphosis, and diapause termination in insects by stimulating the prothoracic glands to synthesize and release the molting hormone, ecdysone. Using Autographa californicanucleopolyhedrovirus (AcNPV)-mediated transient gene transfer into the central nervous sytem (CNS) of the silkworm, Bombyx mori, we identified two cis-regulatory elements that participate in the decision and the enhancement ofPTTHgene expression in PTTH-producing neurosecretory cells (PTPCs). . | ềFEBS Journal Myocyte enhancer factor 2 MEF2 is a key modulator of the expression of the prothoracicotropic hormone gene in the silkworm Bombyx mori Kunihiro Shiomi1 Yoshihiro Fujiwara1 Tsutomu Atsumi1 Zenta Kajiura1 Masao Nakagaki1 Yoshiaki Tanaka2 Akira Mizoguchi3 Toshinobu Yaginuma4 and Okitsugu Yamashita4 5 1 Faculty of Textile Science and Technology Shinshu University Nagano Japan 2 NationalInstitute of AgrobiologicalSciences NIAS Ibaraki Japan 3 Graduate Schoolof Science Nagoya University Aichi Japan 4 Graduate Schoolof BioagriculturalSciences Nagoya University Aichi Japan 5 Chubu University Aichi Japan Keywords baculovirus Bombyx mori MEF2 metamorphosis and diapause PTTH Correspondence K. Shiomi Faculty of Textile Science and Technology Shinshu University Ueda Nagano 386-8567 Japan Fax 81 268 21 5331 Tel 81 268 21 5338 E-mail shiomi@ Database The sequences reported in this paper have been deposited in the DDBJ database under Accession no. AB121093. Received 14 April2005 revised 24 May 2005 accepted 31 May 2005 doi Prothoracicotropic hormone PTTH plays a central role in controlling molting metamorphosis and diapause termination in insects by stimulating the prothoracic glands to synthesize and release the molting hormone ecdysone. Using Autographa California nucleopolyhedrovirus AcNPV -mediated transient gene transfer into the central nervous sytem CNS of the silkworm Bombyx mori we identified two cis-regulatory elements that participate in the decision and the enhancement of PTTH gene expression in PTTH-producing neurosecretory cells PTPCs . The cis-element mediating the enhancement of PTTH gene expression binds the transcription factor Bombyx myocyte enhancer factor 2 BmMEF2 . The BmMEF2 gene was expressed in various tissues including the CNS. In brain the BmMEF2 gene was expressed at elevated levels in two types of lateral neurosecretory cells namely PTPCs and corazonin-like immunoreactive lateral .