tailieunhanh - Báo cáo khoa học: Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II

Distribution of photosystem II (PSII) extrinsic proteins was examined using antibodies raised against various extrinsic proteins from different sources. The results showed that a glaucophyte (Cyanophora paradoxa) having the most primitive plastids contained the cyanobacterial-type extrinsic proteins (PsbO, PsbV, PsbU), and the primitive red algae (Cyanidium caldarium) contained the red algal-type extrinsic proteins (PsO, PsbQ¢, PsbV, PsbU), whereas a prasinophyte (Pyraminonas parkeae), which is one of the most primitive green algae, contained the green algal-type ones (PsbO, PsbP, PsbQ) | iFEBS Journal Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II Isao Enami1 Takehiro Suzuki1 Osamu Tada1 Yoshiko Nakada1 Kumi Nakamura1 Akihiko Tohri1 Hisataka Ohta1 Isao Inoue2 and Jian-Ren Shen3 1 Department of Biology Faculty of Science Tokyo University of Science Tokyo Japan 2 Institute of BiologicalScience University of Tsukuba Japan 3 Department of Biology Faculty of Science Okayama University and PRESTO JST Japan Keywords evolution immunologicalassay oxygen evolution photosystem II PSII extrinsic proteins Correspondence I. Enami Department of Biology Faculty of Science Tokyo University of Science Kagurazaka 1-3 Shinjuku-ku Tokyo 162-8601 Japan Tel 81 471241501 ext. 5022 Fax 81 332600322 E-mail enami@ Received 14 June 2005 revised 8 August 2005 accepted 11 August 2005 doi Distribution of photosystem II PSII extrinsic proteins was examined using antibodies raised against various extrinsic proteins from different sources. The results showed that a glaucophyte Cyanophora paradoxa having the most primitive plastids contained the cyanobacterial-type extrinsic proteins PsbO PsbV PsbU and the primitive red algae Cyanidium caldarium contained the red algal-type extrinsic proteins PsO PsbQ . PsbV PsbU whereas a prasinophyte Pyraminonas parkeae which is one of the most primitive green algae contained the green algal-type ones PsbO PsbP PsbQ . These suggest that the extrinsic proteins had been diverged into cyanobacterial- red algal- and green algal-types during early phases of evolution after a primary endosymbiosis. This study also showed that a hapto-phyte diatoms and brown algae which resulted from red algal secondary endosymbiosis contained the red algal-type whereas Euglena gracilis resulted from green algal secondary endosymbiosis contained the green algal-type extrinsic proteins suggesting that the red algal- and green algal-type .