tailieunhanh - Báo cáo khoa học: The specific delivery of proteins to human liver cells by engineered bio-nanocapsules

A bio-nanocapsule (BNC), composed of the surface antigen (sAg) of the hepatitis B virus, is an efficient nanomachine with which to accomplish the liver-specific delivery of genes and drugs. Approximately 110 molecules of sAg are associated to form a BNC particle with an average diameter of 130 nm. TheLprotein is an sAg peptide composed mainly of preS and S regions. The preS region, with specific affinity for human hepatocytes, is localized in the N-terminus. | ềFEBS Journal The specific delivery of proteins to human liver cells by engineered bio-nanocapsules Dongwei Yu1 Chie Amano1 Takayuki Fukuda1 2 Tadanori Yamada3 Shun ichi Kuroda3 4 Katsuyuki Tanizawa3 4 Akihiko Kondo4 5 Masakazu Ueda4 6 Hidenori Yamada1 Hiroko Tada1 and Masaharu Seno1 4 7 1 Graduate Schoolof NaturalScience and Technology Okayama University Japan 2 Kobe R D Center Katayama ChemicalIndustries Co. Ltd Kobe Japan 3 Institute of Scientific and IndustrialResearch Osaka University Japan 4 Research and Development Division Beacle Inc. Okayama Japan 5 Faculty of Engineering Kobe University Japan 6 Keio University Schoolof Medicine Tokyo Japan 7 Research Center for BiomedicalEngineering Okayama University Japan Keywords bio-nanocapsule L fusion protein protein delivery vector specific infection topology Correspondence M. Seno Graduate Schoolof Natural Science and Technology Okayama University Tsushima-Naka Okayama 700-8530 Japan Tel Fax 81 86 251 8216 E-mail mseno@ Received 20 March 2005 revised 9 May 2005 accepted 24 May 2005 doi A bio-nanocapsule BNC composed of the surface antigen sAg of the hepatitis B virus is an efficient nanomachine with which to accomplish the liver-specific delivery of genes and drugs. Approximately 110 molecules of sAg are associated to form a BNC particle with an average diameter of 130 nm. The L protein is an sAg peptide composed mainly of preS and S regions. The preS region with specific affinity for human hepatocytes is localized in the N-terminus. The S region following the preS has two transmembrane regions responsible for the formation of particles. In this study the fusion of emerald green fluorescent protein EGFP at the C-terminus of the S region was designed to deliver proteins to human hepatocytes. Truncation of the C-terminus of the S region was required to obtain sufficient expression levels in Cos7 cells. The nanoparticles that were produced delivered EGFP to .

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