tailieunhanh - Báo cáo khoa học: 6-Phosphofructo-2-kinase and fructose-2,6-bisphosphatase in Trypanosomatidae Molecular characterization, database searches, modelling studies and evolutionary analysis

Fructose 2,6-bisphosphate is a potent allosteric activator of trypanosomatid pyruvate kinase and thus represents an important regulator of energy meta-bolism in these protozoan parasites. A 6-phosphofructo-2-kinase, respon-sible for the synthesis of this regulator, was highly purified from the bloodstream form ofTrypanosoma bruceiand kinetically characterized. By searching trypanosomatid genome databases, four genes encoding proteins homologous to the mammalian bifunctional enzyme 6-phosphofructo-2-kinase⁄fructose-2,6-bisphosphatase (PFK-2⁄FBPase-2) were found for both T. . | iFEBS Journal 6-Phosphofructo-2-kinase and fructose-2 6-bisphosphatase in Trypanosomatidae Molecular characterization database searches modelling studies and evolutionary analysis Nathalie Chevalier1 Luc Bertrand2 3 Mark H. Rider2 Fred R. Opperdoes1 Daniel J. Rigden4 and Paul A. M. Michels1 1 Research Unit for TropicalDiseases Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry Universite catholique de Louvain Brussels Belgium 2 Hormone and Metabolic Research Unit Christian de Duve Institute of Cellular Pathology and Laboratory of Biochemistry Universite catholique de Louvain Brussels Belgium 3 Division of Cardiology Universite catholique de Louvain Brussels Belgium 4 Schoolof BiologicalSciences University of Liverpool UK Keywords ankyrin-repeat motif fructose 2 6-bisphosphate glycolysis regulation 6-phosphofructo-2-kinase fructose-2 6-bisphosphatase trypanosome Correspondence P. A. M. Michels ICP-TROP Avenue Hippocrate 74 B-1200 Brussels Belgium Fax 32 27 62 68 53 Tel 32 27 64 74 73 E-mail michels@ These authors contributed equally to this work Database Nucleotide sequence data are available in the DDJB EMBL GenBank databases under accession numbers AY571277 Tb4 AY571278 Tb1 and AY999068 Tb2 Received 7 April2005 revised 10 May 2005 accepted 16 May 2005 doi Fructose 2 6-bisphosphate is a potent allosteric activator of trypanosomatid pyruvate kinase and thus represents an important regulator of energy metabolism in these protozoan parasites. A 6-phosphofructo-2-kinase responsible for the synthesis of this regulator was highly purified from the bloodstream form of Trypanosoma brucei and kinetically characterized. By searching trypanosomatid genome databases four genes encoding proteins homologous to the mammalian bifunctional enzyme 6-phosphofructo-2-kinase fructose-2 6-bisphosphatase PFK-2 FBPase-2 were found for both T. brucei and the related parasite Leishmania major and four pairs .