tailieunhanh - Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB

Members of atRNA Gly 1 multigene family from the silkwormBombyx mori have been classified based on their transcriptions in homologous nuclear extracts, into three groups of highly, moderately and poorly transcribed genes. Because all these gene copies have identical coding sequences and consequently identical promoter elements (the A and B boxes), the flanking sequences modulate their expression levels. Here we demonstrate the inter-action of transcription factor TFIIIB with these genes and its role in regu-lating differential transcriptions | ềFEBS Journal Transcription of individual tRNAGv genes from within a multigene family is regulated by transcription factor TFIIIB Akhila Parthasarthy and Karumathil P. Gopinathan Department of Microbiology and Cell Biology Indian Institute of Science Bangalore India Keywords Bombyx mori differential transcription RNA pol III transcriptionalregulation transcription factors Correspondence K. P. Gopinathan Department of Microbiology and Cell Biology Indian Institute of Science Bangalore 560012 India Fax 91 80 2360 2697 Tel 91 80 2360 0090 E-mail kpg@ Received 15 June 2005 revised 20 July 2005 accepted 25 July 2005 doi Members of a tRNAjGly multigene family from the silkworm Bombyx mori have been classified based on their transcriptions in homologous nuclear extracts into three groups of highly moderately and poorly transcribed genes. Because all these gene copies have identical coding sequences and consequently identical promoter elements the A and B boxes the flanking sequences modulate their expression levels. Here we demonstrate the interaction of transcription factor TFIIIB with these genes and its role in regulating differential transcriptions. The binding of TFIIIB to the poorly transcribed gene tRNAjGly-6 7 was less stable compared with binding of TFIIIB to the highly expressed copy tRNAGly -1. The presence of a 5 upstream TATA sequence closer to the coding region in tRNAGly-6 7 suggested that the initial binding of TFIIIC to the A and B boxes sterically hindered anchoring of TFIIIB via direct interactions leading to lower stability of TFIIIC-B-DNA complexes. Also the multiple TATATAA sequences present in the flanking regions of this poorly transcribed gene successfully competed for TFIIIB reducing transcription. The transcription level could be enhanced to some extent by supplementation of TFIIIB but not by TATA box binding protein. The poor transcription of tRNAGly-6 7 was thus attributed both to the .

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