tailieunhanh - Báo cáo khoa học: The protein tyrosine phosphatase PTP-Basophil/Basophil-like Interacting proteins and molecular functions

The protein tyrosine phosphatase PTP-Basophil (PTP-Bas) and itsmouse homologue, PTP-Basophil-like (PTP-BL), are high molecular mass protein phosphatases consisting of a number of diverse protein–protein interaction modules. Several splicing variants of these phosphatases are known to exist thus demonstrating the complexity of these molecules. PTP-Bas/BL serves as a central scaffolding protein facilita-ting the assembly of a multiplicity of different proteins mainly via five different PDZ domains. . | Eur. J. Biochem. 270 4789-4798 2003 FEBS 2003 doi REVIEW ARTICLE The protein tyrosine phosphatase PTP-Basophil Basophil-like Interacting proteins and molecular functions Kai S. Erdmann Department of Molecular Neurobiochemistry Ruhr-University Bochum Germany The protein tyrosine phosphatase PTP-Basophil PTP-Bas and its mouse homologue PTP-Basophil-like PTP-BL are high molecular mass protein phosphatases consisting of a number of diverse protein-protein interaction modules. Several splicing variants of these phosphatases are known to exist thus demonstrating the complexity of these molecules. PTP-Bas BL serves as a central scaffolding protein facilitating the assembly of a multiplicity of different proteins mainly via five different PDZ domains. Many of these interacting proteins are implicated in the regulation of the actin cytoskeleton. However some proteins demonstrate a nuclear function of this protein tyrosine phosphatase. PTP-Bas is involved in the regulation of cell surface expression of the cell death receptor Fas. Moreover it is a negative regulator of ephrinB phosphorylation a receptor playing an important role during development. The phosphorylation status of other proteins such as RIL IKBa and b-catenin can also be regulated by this phosphatase. Finally PTP-BL has been shown to be involved in the regulation of cytokinesis the last step in cell division. Although the precise molecular function of PTP-Bas BL is still elusive current data suggest clearly that PTP-Bas BL belongs to the family of PDZ domain containing proteins involved in the regulation of the cytoskeleton and of intracellular vesicular transport processes. Keywords cytoskeleton apoptosis phosphatase trafficking cytokinesis. Introduction Protein phosphorylation is one of the most prominent post-translational modifications regulating the activity interaction capability and subcellular localization of proteins. In particular protein tyrosine phosphorylation has .