tailieunhanh - Báo cáo khoa học: Regulation of maize lysine metabolism and endosperm protein synthesis by opaque and floury mutations

The capacity of two maize opaque endosperm mutants (o1ando2) and two floury (fl1andfl2) to accumulate lysine in the seed in relation to their wild type counterparts Oh43+was examined. The highest total lysine content was in theo2mutant and the lowest infl1,as compared with the wild type (). For soluble lysine,o2 exhibitedover a700%increase,whilst for fl3a28%decrease was encountered, as comparedwith thewild type. | Eur. J. Biochem. 270 4898-4908 2003 FEBS 2003 doi Regulation of maize lysine metabolism and endosperm protein synthesis by opaque and floury mutations Ricardo A Azevedo1 Catherine Damerval2 Jacaues Landrv3. Peter J Lea4. Claudia M. Bellato5 BB I B Lyndel Wb Meinhardt6 Martine Le Guilloux2 Sonia Delhaye3 Alejandro Ab Toro1 Salete Ab Gaziola1 and Bertha Db Ab Berdejo1 1 Departamento de Genetica Escola Superior de Agricultura Luiz de Queiroz Universidade de Sao Paulo Sao Paulo Brazil 2Station de Génétique Vegetale INRA UPS INA-PG CNRS UMR 8120 La Ferme du Moulon Gif-sur-Yvette 3INRA Laboratoire de Chimie Biologique INA-PG F78850 Thiverval-Grignon France 4Department of Biological Sciences University of Lancaster Lancaster UK 5Centro de Energia Nuclear na Agricultura Universidade de Sao Paulo Sao Paulo Brazil and 6Departamento de Genetica e Evolupão Universidade Estadual de Campinas Campinas Brazil The capacity of two maize opaque endosperm mutants o1 and o2 and two floury fll and fl2 to accumulate lysine in the seed in relation to their wild type counterparts Oh43 was examined. The 11 i ghett total lysine content was in the o2 mutant and the lowest in fll as compared with the wild type . For soluble lysine o2 exhibited over a 700 increase whilst for fl3 a 28 decrease was encountered as compared with the wild type. In order to understand the mechanisms causini these larie variations in both total and soluble lysine content a quantitative and qualitative study of the N constituents of the endosperm has been carried out and data obtained for the total protein nonprotein N soluble amino acids albumins ilobulins zeins and glutelins present in the seed of the mutants. Following two-dimensional PAGE separation a total of 35 different forms of zein polypeptides were detected and considerable differences were noted between the five different lines. In addition two enzymes of the aspartate biosynthetic pathway aspartate kinase and

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