tailieunhanh - Báo cáo khoa học: Calcium-induced contraction of sarcomeres changes the regulation of mitochondrial respiration in permeabilized cardiac cells

The relationships between cardiac cell structure and the regulation of mitochondrial respiration were studied by applying fluorescent confocal microscopy and analysing the kinetics of mitochondrial ADP-stimulated respiration, during calcium-induced contraction in permeabilized cardiomyo-cytes and myocardial fibers, and in their ‘ghost’ preparations (after selective myosin extraction). | ềFEBS Journal Calcium-induced contraction of sarcomeres changes the regulation of mitochondrial respiration in permeabilized cardiac cells Tiia Anmann1 Margus Eimre2 Andrey V. Kuznetsov3 4 Tatiana Andrienko3 Tuuli Kaambre1 PooÝor Qilzlz1 Fw lin Qzmfwt2 TĨ i ol1 2 l o rpi-r X onzHoli n3 5 F n n Qonncit1 Q nrl Id 11 r A t alvc1 3 Peeter Sikk Evelin Seppet Toomas Tiivel Marko Vendelin Enn Seppet and Valdur A. Saks 1 Laboratory of Bioenergetics National institute of ChemicalPhysics and Biophysics Tallinn Estonia 2 Department of Pathophysiology University of Tartu Estonia 3 Laboratory of Fundamentaland Applied Bioenergetics INSERM E0221 Joseph Fourier University Grenoble France 4 Department of Generaland Transplant Surgery Innsbruck MedicalUniversity Austria 5 Institute of Cybernetics Tallinn Estonia Keywords adenine nucleotides calcium cardiomyocytes intracellular energetic units mitochondria Correspondence V. A. Saks Laboratory of Bioenergetics Joseph Fourier University 2280 Rue de la Piscine BP53X - 38041 Grenoble Cedex 9 France Fax 33 4 76514218 Tel 33 4 76635627 E-mail Received 15 March 2005 revised 21 April 2005 accepted 22 April 2005 doi The relationships between cardiac cell structure and the regulation of mitochondrial respiration were studied by applying fluorescent confocal microscopy and analysing the kinetics of mitochondrial ADP-stimulated respiration during calcium-induced contraction in permeabilized cardiomyocytes and myocardial fibers and in their ghost preparations after selective myosin extraction . Up to 3 pM free calcium in the presence of ATP induced strong contraction of permeabilized cardiomyocytes with intact sarcomeres accompanied by alterations in mitochondrial arrangement and a significant decrease in the apparent Km for exogenous ADP and ATP in the kinetics of mitochondrial respiration. The Vmax of respiration showed a moderate 50 increase with an optimum at IM free calcium .

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