tailieunhanh - Báo cáo khoa học: Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response

The innate antiviral response requires the induction of genes and proteins with activities that limit virus replication. Among these, the well-character-ized interferon b (IFNB) gene is regulated through the cooperation of AP-1, NF-jB and interferon regulatory factor 3 (IRF-3) transcription fac-tors. Using a constitutively active form of IRF-3, IRF-3 5D, we showed previously that IRF-3 also regulates an IFN-independent antiviral response through the direct induction of IFN-stimulated genes. | iFEBS Journal Regulation of arginase II by interferon regulatory factor 3 and the involvement of polyamines in the antiviral response Nathalie Grandvaux1 2 Francois Gaboriau3 Jennifer Harris1 4 Benjamin R. tenOever1 2 Rongtuan Lin4 and John Hiscott1 2 4 1 Terry Fox Molecular Oncology Group Lady Davis Institute for MedicalResearch Montreal Canada 2 Department of Medicine and Oncology McGillUniversity Montreal Canada 3 INSERM U522 Regulations des Equilibres Fonctionnels du Foie Normaland Pathologique CHRU Pontchaillou Rennes France 4 Department of Microbiology and Immunology McGillUniversity Montreal Canada Keywords antiviral response arginase II interferon regulatory factor 3 IRF-3 polyamine spermine Correspondence J. Hiscott Molecular Oncology Group Lady Davis Institute for MedicalResearch 3755 chemin de la Cote Sainte Catherine Montreal Quebec Canada H3T1E2 Fax 514 340 7576 Tel 514 340 8222 Ext. 5265 E-mail Received 11 December 2004 revised 6 April 2005 accepted 20 April 2005 doi The innate antiviral response requires the induction of genes and proteins with activities that limit virus replication. Among these the well-characterized interferon b IFNB gene is regulated through the cooperation of AP-1 NF-kB and interferon regulatory factor 3 IRF-3 transcription factors. Using a constitutively active form of IRF-3 IRF-3 5D we showed previously that IRF-3 also regulates an IFN-independent antiviral response through the direct induction of IFN-stimulated genes. In this study we report that the arginase II gene Argii as well as ArgII protein concentrations and enzymatic activity are induced in IRF-3 5D-expressing and Sendai virus-infected Jurkat cells in an IFN-independent manner. ArgII is a critical enzyme in the polyamine-biosynthetic pathway. Of the natural polyamines spermine possesses antiviral activity and mediates apoptosis at physiological concentrations. Measurement of intracellular polyamine content .