tailieunhanh - Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L.

Interleukin (IL)-10 was cloned from the common carp (Cyprinus carpioL.) using IL-10 primers from carp head kidney following stimulation with concanavalin A and lipopolysaccharide. The cDNA consisted of a 1096 bp se-quence containing a 55 bp 5¢ untranslated region and a 498 bp 3¢ untranslated region. An open reading frame of 543 bp encoded a putative 180 amino acid protein with a putative signal peptide of 22 amino acids. | Eur. J. Biochem. 270 4647-4654 2003 FEBS 2003 doi Cloning characterization and expression analysis of interleukin-10 from the common carp Cyprinus carpio L. Ram Savan1 Daisuke Igawa2 and Masahiro Sakai2 1 United Graduate School of Agricultural Sciences Kagoshima University Korimoto Japan 2Faculty of Agriculture Miyazaki University Miyazaki Japan Interleukin IL -10 was cloned from the common carp Cyprinus carpio L. using IL-10 primers from carp head kidney following stimulation with concanavalin A and lipopolysaccharide. The cDNA consisted of a 1096 bp sequence containing a 55 bp 5 untranslated region and a 498 bp 3 untranslated region. An open reading frame of 543 bp encoded a putative 180 amino acid protein with a putative signal peptide of 22 amino acids. The signature motif of IL-10 is conserved in carp sequence. A 2083 bp genomic sequence of carp IL-10 was found to contain five exons interrupted by four introns. With the exception of much more compact introns the genomic structure was similar to that of mammalian IL-10. By homology phylogeny and genomic analyses the carp gene cloned was designated as IL-10. Carp IL-10 was expressed in head kidney liver spleen and intestine during the resting phase. The gene was also expressed in head kidney and liver following in vitro stimulation with lipopolysaccharide. Keywords cytokines interleukin innate immunity fish expression analysis. Cytokines play a significant role in initiating and regulating the inflammatory process which is an important defense system in innate immunity. Cytokines are subdivided into families such as interleukins ILs lymphokines growth factors interferons IFNs and chemokines. IL-10 initially known as cytokine synthesis inhibitory factor is a multifunctional cytokine and demonstrates immunosuppressive function. The main function of IL-10 seems to be regulation of immunity and the inflammatory response thereby minimizing damage to the host induced by response to a .