tailieunhanh - Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography

Essential oils (also called volatile oils) generally extracted by distillation from plants are hydrophobic liquids containing volatile aromatic compounds. The essential oils are widely used in perfumes, cosmetics as well as in food and drink as flavor additives. Some essential oils show multiple pharmacological activities and have been considered as the major active fractions of herbal medicines [1–3]. Preparative separation of the volatile components is very important not only for the quality control of the crude herbs and the products containing the essential oil but also for the bio-evaluation. However, preparative separation of these pure volatile components is a challenge because of their structural similarity, strongly hydrophobic properties and poor stability. Column. | 1658 J. Sep. Sci. 2010 33 1658-1664 Yuan-Ye Dang Xiao-Cen Li Qing-Wen Zhang Shao-Ping Li Yi-Tao Wang Research Article Preparative isolation and purification of six volatile compounds from essential oil of Institute of Chinese Medical Sciences University of Macau Taipa Macau SAR P. R. China Curcuma wenyujin using high-performance centrifugal partition chromatography Received June 29 2009 Revised January 20 2010 Accepted February 23 2010 Six volatile compounds curdione 1 curcumol 2 germacrone 3 curzerene 4 1 8-cineole 5 and P-elemene 6 were successfully isolated from the essential oil of Curcuma wenyujin by high-performance centrifugal partition chromatography using a nonaqueous two-phase solvent system consisting of petroleum ether-acetonitrile-acetone 4 3 1 v v v . A total of 8 mg of curdione 1 4 mg of curcumol 2 10 mg of germacrone 3 18 mg of curzerene 4 9 mg of 1 8-cineole 5 and 17 mg of P-elemene 6 were isolated from the essential oil 300 mg in 500 min. Their structures were determined by comparison of their retention times and MS data with those of the authentic samples as well as NMR spectroscopic analysis. Keywords Curcuma wenyujin High-performance centrifugal partition chromatography Preparative separation Volatile components Doi 1 Introduction Essential oils also called volatile oils generally extracted by distillation from plants are hydrophobic liquids containing volatile aromatic compounds. The essential oils are widely used in perfumes cosmetics as well as in food and drink as flavor additives. Some essential oils show multiple pharmacological activities and have been considered as the major active fractions of herbal medicines 1-3 . Preparative separation of the volatile components is very important not only for the quality control of the crude herbs and the products containing the essential oil but also for the bio-evaluation. However preparative separation of these pure volatile components is a challenge because of their .