tailieunhanh - Báo cáo khoa học: C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition

By binding to the CArG box sequence, the serum response factor (SRF) activates several muscle-specific genes, as well as genes that respond to mitogens. The core domain of the SRF (core-SRF) binds as a dimer to the CArG box C)5C)4A)3T)2A)1T+1T+2A+3G+4G+5of the c-fos serum response element (SRE fos ). | ỊFEBS Journal C fi G base mutations in the CArG box of c-fos serum response element alter its bending flexibility Consequences for core-SRF recognition Josef Stepanek1 2 z Michel Vincent3 f Pierre-Yves Turpin1 Denise Paulin2 Serge Fermandjian4 5 Bernard Alpert2 and Christian Zentz1 1 Laboratoire de Biophysique Moleculaire Cellulaire Tissulaire Universite Pierre et Marie Curie Evry France 2 Laboratoire de Biologie Moleculaire de la Differenciation Universite Denis Diderot Paris France 3 LURE Universite Paris-Sud Orsay France 4 Departement de Biologie et Pharmacologie Structurales Ecole Normale Superieure de Cachan France 5 Institut Gustave Roussy Villejuif France Keywords CArG box c-fos DNA bending DNA dynamics serum response element Correspondence C. Zentz Laboratoire de Biophysique Moleculaire Cellulaire Tissulaire Universite Pierre et Marie Curie CNRS UMR 7033 GENOPOLE Campus 1 5 rue Henri Desbrueres 91030 Evry Cedex France Fax 33 1 69 87 43 60 Tel 33 1 69 87 43 52 E-mail zentz@ Permanent address Charles University Faculty of Mathematics and Physics Prague Czech Republic Present address IBBMC Universite Paris-Sud Orsay France Received 22 December 2006 revised 20 February 2007 accepted 2 March 2007 doi By binding to the CArG box sequence the serum response factor SRF activates several muscle-specific genes as well as genes that respond to mitogens. The core domain of the SRF core-SRF binds as a dimer to the CArG box C_5C_4A_3T_2A_1T 1T 2A 3G 4G 5 of the c-fos serum response element SREfos . However previous studies using 20-mer DNAs have shown that the binding stoichiometry of core-SRF is significantly altered by mutations C_5 fi G SREGfos and C_5C_4 fi GG SREGGfos of the CArG box A Huet A Parlakian M-C Arnaud J-M Glandieres. P Valat S Fermandjian D Paulin B Alpert C Zentz 2005 FEBS J 272 3105-3119 . To understand these effects we carried out a comparative analysis of the three 20-mer DNAs SREfos SREGfos and SREGGfos

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