tailieunhanh - Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family

DESC1 was identified using gene-expression analysis between squamous cell carcinoma of the head and neck and normal tissue. It belongs to the type II transmembrane multidomain serine proteinases (TTSPs), an expanding family of serine proteinases, whose members are differentially expressed in several tissues. | ễFEBS Journal Crystal structure of the catalytic domain of DESC1 a new member of the type II transmembrane serine proteinase family I p l t ioIoỉQ 1 Rohot Ý I I 11 hr f1 2 Fflfl9 f f nri3 R 3n f iohlot 3 I 111 ntof3 OLLO J. r. Kyiieieis nuueiL nuuei Luyai Oily nyan oeniei Mike nuiHei Edwin L. Madison3 and Uwe Jacob1 f 1 Max-Planck-Institut fur Biochemie Martinsried Germany 2 Cardiff University UK 3 Corvas International San Diego CA USA Keywords squamous cell carcinoma of the head and neck trypsin-like serine protease tumor marker type II transmembrane serine proteinases Correspondence O. J. P. Kyrieleis Max-Planck-Institut fur Biochemie Abteilung Strukturforschung Am Klopferspitz 18a D-82152 Martinsried Germany Fax 33 0476207199 Tel 33 0476207860 E-mail kyrieleis@ Present address EMBL Grenoble Outstation France ỊSuppreMolGmbH Martinsried Germany Database The coordinates and structure factors for DESC1-benzamidine complex have been deposited in the RCSB Protein Data Bank under the accession number 2OQ5 Received 17 October 2006 revised 31 January 2007 accepted 26 February 2007 doi DESC1 was identified using gene-expression analysis between squamous cell carcinoma of the head and neck and normal tissue. It belongs to the type II transmembrane multidomain serine proteinases TTSPs an expanding family of serine proteinases whose members are differentially expressed in several tissues. The biological role of these proteins is currently under investigation although in some cases their participation in specific functions has been reported. This is the case for enteropeptidase hepsin matriptase and corin. Some members including DESC1 are associated with cell differentiation and have been described as tumor markers. TTSPs belong to the type II transmembrane proteins that display in addition to a C-terminal trypsin-like serine proteinase domain a differing set of stem domains a transmembrane segment and a short N-terminal .