tailieunhanh - Báo cáo khoa học: Gliadain, a gibberellin-inducible cysteine proteinase occurring in germinating seeds of wheat, Triticum

We cloned a new cysteine proteinase of wheat seed origin, which hydro-lyzed the storage protein gliadin almost specifically, and was named glia-dain. Gliadain mRNA was expressed 1 day after the start of seed imbibition, and showed a gradual increase thereafter. | ễFEBS Journal Gliadain a gibberellin-inducible cysteine proteinase occurring in germinating seeds of wheat Triticum aestivum L. specifically digests gliadin and is regulated by intrinsic cystatins Toshihiro Kiyosaki1 Ichiro Matsumoto1 Tomiko Asakura1 2 Junko Funaki3 Masaharu Kuroda4 Takumi Misaka1 Soichi Arai5 and Keiko Abe1 1 Department of Applied BiologicalChemistry Graduate Schoolof Agriculturaland Life Sciences The University of Tokyo Japan 2 Faculty of Management Atomi University Saitama Japan 3 Faculty of Human EnvironmentalScience Fukuoka Women s University Japan 4 NationalAgriculturalResearch Center Niigata Japan 5 Department of NutritionalScience Tokyo University of Agriculture Japan Keywords cystatin cysteine proteinase gibberellin gliadin wheat Correspondence K. Abe Department of Applied Biological Chemistry Graduate Schoolof Agricultural and Life Sciences The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan Fax 81 3 5841 8006 Tel 81 3 5841 5129 E-mail aka7308@ T. Asakura Faculty of Management Atomi University 1-9-6 Nakano Niiza-shi Saitama 352-8501 Japan Fax 81 4 8478 4142 Tel 81 4 8478 4110 E-mail asakura@ Received 9 January 2007 revised 10 February 2007 accepted 19 February 2007 doi We cloned a new cysteine proteinase of wheat seed origin which hydrolyzed the storage protein gliadin almost specifically and was named glia-dain. Gliadain mRNA was expressed 1 day after the start of seed imbibition and showed a gradual increase thereafter. Gliadain expression was suppressed when uniconazol a gibberellin synthesis inhibitor was added to germinating seeds. Histochemical detection with anti-gliadain serum indicated that gliadain was present in the aleurone layer and also that its expression intensity increased in sites nearer the embryo. The enzymological characteristics of gliadain were investigated using recombinant glutathione S-transferase GST -progliadain fusion .