tailieunhanh - Báo cáo khoa học: p130-Angiomotin associates to actin and controls endothelial cell shape

Angiomotin, an 80 kDa protein expressed in endothelial cells, promotes cell migration and invasion, and stabilizes tube formationin vitro. Angiomotin belongs to a new protein family with two additional members, Amotl-1 and Amotl-2, which are characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. | iFEBS Journal p130-Angiomotin associates to actin and controls endothelial cell shape Mira Ernkvist1 Karin Aase1 Chinwe Ukomadu2 3 James Wohlschlegel2 Ryan Blackman2 3 Niina Veitonmaki1 Anders Bratt1 Anindya Dutta2 3 4 and Lars Holmgren1 1 Department of Oncology-Pathology Cancer Centre Karolinska Institute Stockholm Sweden 2 Department of Pathology Brigham and Women s Hospital Boston MA USA 3 Department of Medicine Brigham and Women s Hospital Boston MA USA 4 Department of Biochemistry and Molecular Genetics University of Virginia Charlottesville VA USA Keywords actin fiber angiogenesis angiostatin migration tight junction Correspondence L. Holmgren Department of Oncology and Pathology CCK R8 03 Karolinska University Hospital SE-171 76 Stockholm Sweden Fax 46 8 339 031 Tel 46 8 517 79317 E-mail Database The nucleotide sequence reported here has been submitted to the GenBank database under bankit number 694853 and accession number AY987378. Angiomotin an 80 kDa protein expressed in endothelial cells promotes cell migration and invasion and stabilizes tube formation in vitro. Angiomotin belongs to a new protein family with two additional members Amotl-1 and Amotl-2 which are characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. Here we report the identification of a 130 kDa splice isoform of angiomotin that is expressed in different cell types including vascular endothelial cells as well as cytotrophoblasts of the placenta. p130-Angiomotin consists of a cytoplasmic N-terminal extension that mediates its association with F-actin. Transfection of p130-angiomotin into endothelial cells induces actin fiber formation and changes cell shape. The p130-angiomotin protein remained associated with actin after destabilization of actin fibers with cytochalasin B. In contrast to p80-angiomotin p130-angiomotin does not promote cell migration and did not respond to angiostatin. We propose that p80- and p130-angiomotin play .