tailieunhanh - Báo cáo khoa học: Towards understanding the functional role of the glycosyltransferases involved in the biosynthesis of Moraxella catarrhalis lipooligosaccharide

The glycosyltransferase enzymes (Lgts) responsible for the biosynthesis of the lipooligosaccharide-derived oligosaccharide structures from Moraxella catarrhalishave been investigated. This upper respiratory tract pathogen is responsible for a spectrum of illnesses, including otitis media (middle ear infection) in children, and contributes to exacerbations of chronic obstruct-ive pulmonary disease in elderly patients. | ễFEBS Journal Towards understanding the functional role of the glycosyltransferases involved in the biosynthesis of Moraxella catarrhalis lipooligosaccharide Ian R. Peak1 I. D. Grice1 Isabelle Faglin1 Zoran Klipic1 Patrick M. Collins1 Lucien van Schendel1 Paul G. Hitchen2 Howard R. Morris3 Anne Dell2 and Jennifer C. Wilson1 1 Institute for Glycomics Griffith University Gold Coast Campus Queensland Australia 2 Division of Molecular Biosciences Faculty of NaturalSciences ImperialCollege London UK 3 M-SCAN Mass Spectrometry Research and Training Centre Silwood Park Ascot UK Keywords glycosyltransferase lipooligosaccharide biosynthesis Moraxella catarrhalis MS NMR spectroscopy Correspondence J. C. Wilson Institute for Glycomics Griffith University Gold Coast Campus PMB 50 QLD 4215 Australia Fax 61 7 555 28908 Tel 61 7 555 28077 E-mail Database Sequences have been deposited under the accession numbers DQ071425 2951 locus DQ071426 3292 locus and DQ071427-DQ071431 lgt2 alleles Received 24 August 2006 revised 29 January 2007 accepted 16 February 2007 doi The glycosyltransferase enzymes Lgts responsible for the biosynthesis of the lipooligosaccharide-derived oligosaccharide structures from Moraxella catarrhalis have been investigated. This upper respiratory tract pathogen is responsible for a spectrum of illnesses including otitis media middle ear infection in children and contributes to exacerbations of chronic obstructive pulmonary disease in elderly patients. To investigate the function of the glycosyltransferase enzymes involved in the biosynthesis of lipooligosaccha-ride of M. catarrhalis and to gain some insight into the mechanism of serotype specificity for this microorganism mutant strains of M. catarrhalis were produced. Examination by NMR and MS of the oligosaccharide structures produced by double-mutant strains 2951lgt1 4A and 2951lgt5 4A and a single-mutant strain 2951lgt2D of the bacterium has .