tailieunhanh - Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells

We characterized the expression and functional properties of the ADP-sensitive P2Y1 and P2Y12 nucleotide receptors in glioma C6 cells cultured in medium devoid of serum for up to 96 h. During this long-term serum starvation, cell morphology changed from fibroblast-like flat to round, the adhesion pattern changed, cell-cycle arrest was induced, | ễFEBS Journal Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells Patryk Krzeminski Dorota Suptat Rafat Czajkowski Pawet Pomorski and Jolanta Baranska Department of Molecular and Cellular Neurobiology Nencki Institute of ExperimentalBiology Polish Academy of Sciences Warsaw Poland Keywords Akt glioma C6 cells mitogen-activated protein kinase nucleotide receptors serum deprivation Correspondence J. Baraitska Department of Molecular and Cellular Neurobiology Nencki Institute of ExperimentalBiology Polish Academy of Sciences 3 Pasteur Street Warsaw PL 02-093 Poland Fax 48 22 8225342 Tel 48 22 5892300 E-mail Received 6 June 2006 revised 7 February 2007 accepted 14 February 2007 doi We characterized the expression and functional properties of the ADP-sensitive P2Y1 and P2Y12 nucleotide receptors in glioma C6 cells cultured in medium devoid of serum for up to 96 h. During this long-term serum starvation cell morphology changed from fibroblast-like flat to round the adhesion pattern changed cell-cycle arrest was induced extracellular signal-regulated kinase ERK1 2 phosphorylation was reduced Akt phosphorylation was enhanced and expression of the P2Y12 receptor relative to P2Y1 was increased. These processes did not reflect differentiation into astrocytes or oligodendrocytes as expression of glial fibrillary acidic protein and NG2 proteoglycan standard markers of glial cell differentiation was not increased during the serum deprivation. Transfer of the cells into fresh medium containing 10 fetal bovine serum reversed the changes. This demonstrates that serum starvation caused only temporary growth arrest of the glioma C6 cells which were ready for rapid division as soon as the environment became more favorable. In cells starved for 72 and 96 h expression of the P2Y1 receptor was low and the P2Y12 receptor was the major player responsible for .

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