tailieunhanh - Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2

Eight phospholipases A2 (PLAs) and four three-finger-toxins (3FTx) from the pooled venom of Bungarus fasciatus(Bf) were previously studied and sequenced, but their expression pattern in individual Bf venom and possible geographic variations remained to be investigated. We herein analyze the individual venom of two Bf specimens from Kolkata (designated as KBf) to address this question. | ễFEBS Journal Sequences geographic variations and molecular phylogeny of venom phospholipases and threefinger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 Inn-Ho Tsai1 Hsin-Yu Tsai1 Archita Saha2 and Antony Gomes2 1 Institute of BiologicalChemistry Academia Sinica Taiwan Institute of BiochemicalSciences NationalTaiwan University Taipei Taiwan 2 Department of Physiology University of Calcutta Kolkata India Keywords Bungarus fasciatus cDNA cloning phospholipase A2 phylogenetic analysis threefinger toxins venom geographic variation Correspondence . Tsai Institute of BiologicalChemistry Academia Sinica Taiwan Institute of BiochemicalSciences NationalTaiwan University POB 23-106 Taipei Taiwan Fax 886 223635038 E-mail bc201@ Database The sequence data were deposited in the GenBank database with the accession numbers DQ508406 DQ508411-14 for KBf-VI KBf-grIB KBf-II KBf-Va and KBf-X DQ768745 for KBf-III DQ835584 for Vb-2 respectively DQ508407-10 for 3FTx-LI -LK -RK and -RI and DQ835582-3 for VIIIa and 3FTx-LT respectively Received 30 June 2006 revised 16 October 2006 accepted 17 November 2006 doi Eight phospholipases A2 PLAs and four three-finger-toxins 3FTx from the pooled venom of Bungarus fasciatus Bf were previously studied and sequenced but their expression pattern in individual Bf venom and possible geographic variations remained to be investigated. We herein analyze the individual venom of two Bf specimens from Kolkata designated as KBf to address this question. Seven PLAs and five 3FTx were purified from the KBf venoms and respective cDNAs were cloned from venom glands of one of the snakes. Comparison of their mass and N-terminal sequence revealed that all the PLAs were conserved in both KBf venoms but that two of their 3FTx isoforms were variable. When comparing the sequences of these KBf-PLAs with those published only one was found to be identical to that of Bf