tailieunhanh - Báo cáo khoa học: cGMP transport by vesicles from human and mouse erythrocytes

cGMP secretion from cells can be mediated by ATP-binding cassette (ABC) transporters ABCC4, ABCC5, and ABCC11. Indirect evidence sug-gests that ABCC4 and ABCC5 contribute to cGMP transport by erythro-cytes. We have re-investigated the issue using erythrocytes from wild-type and transporter knockout mice. | ỊFEBS Journal cGMP transport by vesicles from human and mouse erythrocytes Cornelia J. F. de Wolf1 Hiroaki Yamaguchi1 Ingrid van der Heijden1 Peter R. Wielinga1 Stefanie L. Hundscheid1 Nobuhito Ono1 George L. Scheffer2 Marcel de Haas1 John D. Schuetz3 Jan Wijnholds1 4 and Piet Borst1 1 Department of Molecular Biology the Netherlands Cancer Institute Amsterdam the Netherlands 2 Department of Pathology Free University MedicalCenter Amsterdam the Netherlands 3 Department of PharmaceuticalSciences St Jude Children s Research Hospital Memphis TN USA 4 Netherlands Institute for Neurosciences RoyalNetherlands Academy of Arts and Sciences KNAW Amsterdam the Netherlands Keywords ABCC4 ABCG2 cGMP multidrug resistance multidrug resistance protein MRP Correspondence P. Borst Department of Molecular Biology the Netherlands Cancer Institute 1066 CX Plesmanlaan 121 Amsterdam the Netherlands Fax 31 20 6691383 Tel 31 20 5122880 E-mail Present address Department of PharmaceuticalSciences Tohoku University Hospital Sendai Japan TNationallnstitute for Public Health and Environment RIVM Microbiological Laboratory for Health Protection MGB Bilthoven the Netherlands Division of Diagnostic Oncology the Netherlands Cancer Institute Amsterdam the Netherlands The 2nd Department of InternalMedicine Faculty of Medicine Kagoshima University Kagoshima Japan Received 13 September 2006 revised 20 October 2006 accepted 13 November 2006 doi cGMP secretion from cells can be mediated by ATP-binding cassette ABC transporters ABCC4 ABCC5 and ABCC11. Indirect evidence suggests that ABCC4 and ABCC5 contribute to cGMP transport by erythrocytes. We have re-investigated the issue using erythrocytes from wild-type and transporter knockout mice. Murine wild-type erythrocyte vesicles transported cGMP with an apparent Km that was 100-fold higher than their human counterparts the apparent Vmax being similar. Whereas cGMP transport into human vesicles was efficiently