tailieunhanh - Báo cáo khoa học: Aly⁄ REF, a factor for mRNA transport, activates RH gene promoter function

The rhesus (Rh) blood group antigens are of considerable importance in transfusion medicine as well as in newborn or autoimmune hemolytic diseases due to their high antigenicity. We identified a major DNaseI hypersensitive site at the 5¢ flanking regions of bothRHDandRHCEexon 1. A 34 bp fragment located at)191 to)158 from a translation start posi-tion, and containing the TCCCCTCCC sequence, was involved in enhan-cing promoter activity, which was assessed by luciferase reporter gene assay. | iFEBS Journal Aly REF a factor for mRNA transport activates RH gene promoter function Hiroshi Suganuma1 Maki Kumada1 Toshinori Omi1 Takaya Gotoh1 Munkhtulga Lkhagvasuren1 Hiroshi Okuda1 2 Toyomi Kamesaki1 2 Eiji Kajii1 2 and Sadahiko Iwamoto1 1 Division of Human Genetics Center for Community Medicine Jichi MedicalSchool Japan 2 Division of Community Medicine Center for Community Medicine Jichi Medical School Japan Keywords Rh blood group antigen promoter transcription cofactor Aly REF Correspondence S Iwamoto Division of Human Genetics Center for Community Medicine Jichi Medical School 3311-1 Minamikawachi-machi Kawachi-gun Tochigi 329-0498 Japan Fax 81 285 44 49 Tel 81 285 58 7342 E-mail siwamoto@ Note Hiroshi Suganuma and Sadahiko Iwamoto contributed equally to this work The rhesus Rh blood group antigens are of considerable importance in transfusion medicine as well as in newborn or autoimmune hemolytic diseases due to their high antigenicity. We identified a major DNaseI hypersensitive site at the 5 flanking regions of both RHD and RHCE exon 1. A 34 bp fragment located at -191 to -158 from a translation start position and containing the TCCCCTCCC sequence was involved in enhancing promoter activity which was assessed by luciferase reporter gene assay. A biotin-labelled 34 bp probe isolated an mRNA transporter protein Aly REF. The specific binding of Aly REF to RH promoter in erythroid was confirmed by chromatin immunoprecipitation assay. The silencing of Aly REF by siRNA reduced not only the RH promoter activity of the reporter gene but also transcription from the native genome. These facts provide second proof of Aly REF as a transcription coactivator initially identified as a coactivator for the TCRa enhancer function. Aly REF might be a novel transcription cofactor for erythroid-specific genes. Received 26 November 2004 revised 17 March 2005 accepted 23 March 2005 doi The rhesus Rh blood group antigens are of .

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