tailieunhanh - Báo cáo khoa học: Specific TSC22 domain transcripts are hypertonically induced and alternatively spliced to protect mouse kidney cells during osmotic stress

We recently cloned a novel osmotic stress transcription factor 1 (OSTF1) from gills of euryhaline tilapia (Oreochromis mossambicus) and demonstra-ted that acute hyperosmotic stress transiently increases OSTF1 mRNA and protein abundance [Fiol DF, Ku¨ltz D (2005) Proc Natl Acad Sci USA 102, 927–932]. | ỊFEBS Journal Specific TSC22 domain transcripts are hypertonically induced and alternatively spliced to protect mouse kidney cells during osmotic stress Diego F. Fiol Sally K. Mak and Dietmar Kultz PhysiologicalGenomics Group Department of AnimalScience University of California Davis CA USA Keywords aldosterone hyperosmotic stress hypertonicity kidney mIMCD3 cells Correspondence D. Kultz PhysiologicalGenomics Group Department of AnimalScience University of California Davis One Shields Avenue Davis CA 95616 USA Fax 1 530 752 0175 Tel 1 530 752 2991 E-mail dkueltz@ Present address The Parkinson s Institute Sunnyvale CA USA Received 28 July 2006 revised 23 October 2006 accepted 3 November 2006 doi We recently cloned a novel osmotic stress transcription factor 1 OSTF1 from gills of euryhaline tilapia Oreochromis mossambicus and demonstrated that acute hyperosmotic stress transiently increases OSTF1 mRNA and protein abundance Fiol DF Kultz D 2005 Proc Natl Acad Sci USA 102 927-932 . In this study a genome-wide search was conducted to identify nine distinct mouse transforming growth factor TGF -b-stimulated clone 22 domain TSC22D transcripts including glucocorticoid-induced leucine zipper GILZ that are orthologs of OSTF1. These nine TSC22D transcripts are encoded at four loci on chromosomes 14 TSC22D1 two splice variants 3 TSC22D2 four splice variants X TSC22D3 two splice variants and 5 TSC22D4 . All nine mouse TSC22D transcripts are expressed in renal cortex medulla and papilla and in the mIMCD3 cell line. The two TSC22D3 transcripts including GILZ are upregulated by aldosterone but not by hyperosmolality in mIMCD3 cells. In contrast TSC22D4 is stably upregulated by hyperosmolality in mIMCD3 cells and increased in renal papilla compared with cortex. Moreover all four TSC22D2 transcripts are transiently upregulated by hyperosmolality and resemble tilapia OSTF1 in this regard. All TSC22D2 transcripts depend on hypertonicity as .