tailieunhanh - Báo cáo khoa học: Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264.7 murine macrophages

Exposure to quartz particles induces a pathological process named silicosis. Alveolar macrophages initiate the disease through their activation, which is the origin of the later dysfunctions. Ascorbic acid is known to selectively dissolve the quartz surface. During the reaction, ascorbic acid progressively disappears and hydroxyl radicals are generated from the quartz surface. These observations may be relevant to mammalian quartz toxicity, as sub-stantial amounts of ascorbic acid are present in the lung epithelium. . | ễFEBS Journal Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW murine macrophages Sonia Scarfl1 2 Umberto Benatti2 Marina Pozzolini1 2 Emanuela Clavarino2 Chiara Ferraris1 2 Mirko Magnone2 Laura Valisano3 and Marco Giovine1 4 1 Advanced Biotechnology Center Genoa Italy 2 Department of ExperimentalMedicine Section of Biochemistry University of Genoa Italy 3 Department for the Study of the Territory and its Resources University of Genoa Italy 4 Department of Biology University of Genoa Italy Keywords ascorbic acid inflammation macrophages reactive oxygen species silica Correspondence S. Scarfl Department of Experimental Medicine Section of Biochemistry University of Genoa Viale Benedetto XV n 1 16132 Genoa Italy Fax 39 010 354415 Tel 39 010 3538151 E-mail soniascarfl@ Received 22 May 2006 revised 26 October 2006 accepted 30 October 2006 doi Exposure to quartz particles induces a pathological process named silicosis. Alveolar macrophages initiate the disease through their activation which is the origin of the later dysfunctions. Ascorbic acid is known to selectively dissolve the quartz surface. During the reaction ascorbic acid progressively disappears and hydroxyl radicals are generated from the quartz surface. These observations may be relevant to mammalian quartz toxicity as substantial amounts of ascorbic acid are present in the lung epithelium. We studied the inflammatory response of the murine macrophage cell line RAW incubated with ascorbic acid-treated quartz through the expression and activity of the enzyme cyclo-oxygenase-2 COX-2 . COX-2 expression and prostaglandin secretion were enhanced in cells incubated with ascorbic acid-treated quartz. In contrast no changes were observed in cells incubated with Aerosil OX50 an amorphous form of silica. Quantification of COX-2 mRNA showed a threefold increase in cells incubated with ascorbic acid-treated quartz compared with controls. The

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