tailieunhanh - Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops

The 2-hydroxycarboxylate transporter (2HCT) family of secondary trans-porters belongs to a much larger structural class of secondary transporters termed ST3 which contains about 2000 transporters in 32 families. The transporters of the 2HCT family are among the best studied in the class. Here we detect weak sequence similarity between the N- and C-terminal halves of the proteins using a sensitive method which uses a database con-taining the N- and C-terminal halves of all the sequences in ST3 and involves blastsearches of each sequence in the database against the whole database. . | iFEBS Journal Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops Juke S. Lolkema1 Iwona Sobczak1 and Dirk-Jan Slotboom2 1 Molecular Microbiology Biomolecular Sciences and Biotechnology Institute University of Groningen the Netherlands 2 Enzymology Biomolecular Sciences and Biotechnology Institute University of Groningen the Netherlands Keywords domain structure 2-hydroxycarboxylate transporter inverted topology pore-loop structure secondary transporter Correspondence J. S. Lolkema Molecular Microbiology Biomolecular Sciences and Biotechnology Institute University of Groningen Kerklaan 30 9751NN Haren the Netherlands E-mail Received 4 February 2004 revised 10 March 2005 accepted 16 March 2005 doi The 2-hydroxycarboxylate transporter 2HCT family of secondary transporters belongs to a much larger structural class of secondary transporters termed ST3 which contains about 2000 transporters in 32 families. The transporters of the 2HCT family are among the best studied in the class. Here we detect weak sequence similarity between the N- and C-terminal halves of the proteins using a sensitive method which uses a database containing the N- and C-terminal halves of all the sequences in ST3 and involves BLAST searches of each sequence in the database against the whole database. Unrelated families of secondary transporters of the same length and composition were used as controls. The sequence similarity involved major parts of the N- and C-terminal halves and not just a small stretch. The membrane topology of the homologous N- and C-terminal domains was deduced from the experimentally determined topology of the members of the 2HCT family. The domains consist of five transmembrane segments each and have opposite orientations in the membrane. The N terminus of the N-terminal domain is extracellular while the N terminus of the C-ter-minal domain is

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