tailieunhanh - Báo cáo khoa học: Copper-containing nitrite reductase fromPseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center

The nitrite reductase (Nir) isolated fromPseudomonas chlororaphisDSM 50135 is a blue enzyme, with type 1 and type 2 copper centers, as in all copper-containing Nirs des-cribed so far. For the first time, a direct determination of the reduction potentials of both copper centers in a Cu-Nir was performed: type 2 copper (T2Cu), 172 mV and type 1 copper (T1Cu), 298 mV at pH . Although the obtained values seem to be inconsistent with the established electron-transfer mechanism, EPR data indicate that the binding of nitrite to theT2Cu center increases its potential, favoring the electron-transfer process. . | Eur. J. Biochem. 271 2361-2369 2004 FEBS 2004 doi Copper-containing nitrite reductase from Pseudomonas chlororaphis DSM 50135 Evidence for modulation of the rate of intramolecular electron transfer through nitrite binding to the type 2 copper center Dora Pinho1 2 I Stephane Besson2 Carlos D. Brondino2 3 Baltazar de Castro1 and Isabel Moura2 1REQUIMTE Departamento de Quimica Faculdade de Ciências Universidade do Porto Portugal 2REQUIMTE CQFB Departamento de Quimica Faculdade de Ciencias e Tecnologia Universidade Nova de Lisboa Caparica Portugal 3Facultad de Bioquimica y Ciencias Biologicas Universidad Nacional del Litoral Santa Fe Argentina The nitrite reductase Nir isolated from Pseudomonas chlororaphis DSM 50135 is a blue enzyme with type 1 and type 2 copper centers as in all copper-containing Nirs described so far. For the first time a direct determination of the reduction potentials of both copper centers in a Cu-Nir was performed type 2 copper T2Cu 172 mV and type 1 copper T1Cu 298 mV at pH . Although the obtained values seem to be inconsistent with the established electrontransfer mechanism EPR data indicate that the binding of nitrite to the T2Cu center increases its potential favoring the electron-transfer process. Analysis of the EPR spectrum of the turnover form of the enzyme also suggests that the electron-transfer process between T1Cu and T2Cu is the fastest of the three redox processes involved in the catalysis a reduction of T1Cu b oxidation of T1Cu by T2Cu and c reoxidation of T2Cu by NO2-. Electrochemical experiments showthat azurin from the same organism can donate electrons to this enzyme. Keywords copper nitrite reductase EPR redox-titration type 1 copper type 2 copper. Several microorganisms reduce nitrate in a stepwise manner via nitrite to form sequentially NO N2O and eventually dinitrogen as part of their energy-generating metabolism in a process known as denitrification. Nitrite reductase Nir plays a key .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• Structural biochemistry
• oxidation
• bacteria
• N2 fixation
• Antagonistic effect
• Biological control bacteria pseudomonas
• Inhibits free nitrogen fixing bacteria
• Nitrogen fixing bacteria in the rhizosphere
• Potash mobilizing bacteria
• Rabi sorghum
• Yield of rabi sorghum
• Potash mobilizing bacteria on yield
• Phosphate solubilizing bacteria
• Consortium of azotobacter
• Antibacterial activity of bacteria isolated
• Fermenting cocoa water against
• Pathogenic bacteria
• Selected enteropathogenic bacteria
• Under nails
• Identification of bacteria
• Bacteria from under fingernails
• Identify bacteria contaminants
• IAA producing bacteria
• Indole acetic acid producing bacteria
• Coasts of Ben Tre
• Tra Vinh provinces
• Producing bacteria could naturally stimulate
• Colony bacteria
• Isolation of protease producing bacteria
• Protease producing bacteria
• Soil and characterization
• Characterization of the protease
• Yield parameters
• Phosphate solubilising bacteria
• Potash mobilising bacteria inoculants
• Wheat (Triticum aestivum L
• Inorganic fertilizers on yield of wheat
• Lactic acid bacteria
• Bacteriocin production
• Isolation of probiotic bacteria
• Probiotic bacteria
• Optimization of physical
• Nutrition parameters
• Potassium and phosphorus solubilizing bacteria
• Phosphorus solubilizing bacteria
• Characterization of potassium
• Phosphorus solubilising bacteria
• Employing qualitative plating techniques
• Diazotrophic bacteria
• Influence of diazotrophic bacteria
• Diazotrophic bacteria on growth
• Biomass production
• Sugarcane invitro
• Lactic bacteria
• Physiological parameters
• Food fermented
• Lactic bacteria isolated
• Biodiversity of lactic bacteria isolated
• Lactobacillus sporogenes
• Micrococcus sp
• Histology and intestine
• Lactobacillus sporogenes probiotic bacteria
• Thermophile bacteria
• Growth medium
• Medium growth conditions
• Isolation of thermophilic bacteria
• Thermophilic bacteria
• PhosphateSolubilizing Bacteria (PSB)
• Biochemical characterization
• Rhizosphere soil of rice
• Characterization of phosphate solublizing bacteria
• Phosphate solublizing bacteria
• Lactic acid bacteria and yeast
• Standardization of protocol
• Preparation of linseed beverage
• Cultivable diversity
• Fermented fruit mix
• Characterization of probiotic bacteria
• Isolation of chitinase producing bacteria
• Selection of chitinase producing bacteria
• Antifungal activity against fusarium oxysporum from lilium rhizosphere soil
• Lilium rhizosphere soil
• Chitinase producing bacteria
• Endophytic bacteria
• Bacillus sp
• 16S rRNA sequencing
• Endophytic bacteria from tomato
• Molecular characterization of endophytic bacteria
• Plant Associated Bacteria
• Genomics and recent advances
• The endophytic niche
• Plant growth promoting rhizobacteria
• Their ecology and functions
• Nitrogen fixing bacteria in non legumes
• Water Quality
• Indicator bacteria
• Common sources of E
• coli
• Common routes
• Bacteria to streams
• Risks to human health
• Bacterial diversity
• Sea of Marmara
• Culturable bacteria
• Aerobic heterotrophic bacteria
• Genomic DNAs
• Gram positive bacteria
• Gram negative bacteria
• Lecture AP Biology
• Systems biology
• Biological sciences
• Biological organization
• Isolation of calcite precipitation bacteria
• The strength of concrete
• Materials and methods
• Enrichment of Sample and Bacteria Isolation
• Calcium Carbonate Precipitation and Collection
• Causative bacteria
• Urinary tract infection
• Urinary tract infection in children
• The bacteria was resistant
• The third generation of cephalosporines
• Journal of Automation and Control Engineering
• Development and tuning
• Bacteria foraging optimization algorithm
• Cell formation in cellular manufacturing system
• Cellular manufacturing system
• Bacteria foraging optimization algorithm