tailieunhanh - Báo cáo khoa học: Biochemical and spectroscopic characterization of the bacterial phytochrome of Pseudomonas aeruginosa

Phytochromes are photochromic biliproteins found in plants as well as in some cyanotrophic, photoautotrophic and heterotrophic bacteria. In many bacteria, their function is largely unknown. Here we describe the biochemi-cal and spectroscopic characterization of recombinant bacterial phyto-chrome from the opportunistic pathogen Pseudomonas aeruginosa (PaBphP). The recombinant protein displays all the characteristic features of a bonafide phytochrome. | ềFEBS Journal Biochemical and spectroscopic characterization of the bacterial phytochrome of Pseudomonas aeruginosa Ronja Tasler Tina Moises and Nicole Frankenberg-Dinkel Institute for Microbiology TechnicalUniversity Braunschweig Germany Keywords biliverdin histidine kinase linear tetrapyrrole photoreceptor two-component system Correspondence N. Frankenberg-Dinkel Institute for Microbiology TechnicalUniversity Braunschweig Spielmannstr. 7 38106 Braunschweig Germany Fax 49 531 391 5854 Tel 49 531 391 5815 E-mail Received 2 February 2005 revised 17 February 2005 accepted 21 February 2005 doi Phytochromes are photochromic biliproteins found in plants as well as in some cyanotrophic photoautotrophic and heterotrophic bacteria. In many bacteria their function is largely unknown. Here we describe the biochemical and spectroscopic characterization of recombinant bacterial phytochrome from the opportunistic pathogen Pseudomonas aeruginosa PaBphP . The recombinant protein displays all the characteristic features of a bonafide phytochrome. In contrast with cyanobacteria and plants the chromophore of this bacterial phytochrome is biliverdin IXa which is produced by the heme oxygenase BphO in P. aeruginosa. This chromophore was shown to be covalently attached via its A-ring endo-vinyl group to a cysteine residue outside the defined bilin lyase domain of plant and cyano-bacterial phytochromes. Site-directed mutagenesis identified Cys12 and His247 as being important for chromophore binding and photoreversibility respectively. PaBphP is synthesized in the dark in the red-light-absorbing Pr form and immediately converted into a far-red-light-absorbing Pfr-enriched form. It shows the characteristic red far-red-light-induced photoreversibility of phytochromes. A chromophore analog that lacks the C15 16 double bond was used to show that this photoreversibility is due to a 15Z 15E isomerization of the biliverdin chromophore. .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• breast cancer
• medicine
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• oxidation
• tumor cells
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry