tailieunhanh - Báo cáo khoa học: Oxidative stress in the hippocampus after pilocarpineinduced status epilepticus in Wistar rats

The role of oxidative stress in pilocarpine-induced status epilepticus was investigated by measuring lipid peroxidation level, nitrite content, GSH con-centration, and superoxide dismutase and catalase activities in the hippo-campus of Wistar rats. The control group was subcutaneously injected with saline. The experimental group received pilocarpine (400 mgÆkg )1 , subcutaneous). Both groups were killed 24 h after treatment. | ềFEBS Journal Oxidative stress in the hippocampus after pilocarpine-induced status epilepticus in Wistar rats Rivelilson M. Freitas Silvania M. M. Vasconcelos Francisca C. F. Souza Glauce S. B. Viana and Marta M. F. Fonteles Department of Physiology and Pharmacology Laboratory of Neuropharmacology Schoolof Medicine FederalUniversity of Ceara Fortaleza Brazil Keywords hippocampus oxidative stress pilocarpine seizures status epilepticus Correspondence R. M. Freitas Rua Frederico Severo 201 Ap 103 Bl 07 Messejana Fortaleza 60830-310 Brazil Tel Fax 55 85 3274 6091 E-mail rivmendes@ Received 23 October 2004 revised 28 November 2004 accepted 20 December 2004 doi The role of oxidative stress in pilocarpine-induced status epilepticus was investigated by measuring lipid peroxidation level nitrite content GSH concentration and superoxide dismutase and catalase activities in the hippocampus of Wistar rats. The control group was subcutaneously injected with saline. The experimental group received pilocarpine 400 mg kg-1 subcutaneous . Both groups were killed 24 h after treatment. After the induction of status epilepticus there were significant increases 77 and 51 respectively in lipid peroxidation and nitrite concentration but a 55 decrease in GSH content. Catalase activity was augmented 88 but superoxide dismutase activity remained unaltered. These results show evidence of neuronal damage in the hippocampus due to a decrease in GSH concentration and an increase in lipid peroxidation and nitrite content. GSH and catalase activity are involved in mechanisms responsible for eliminating oxygen free radicals during the establishment of status epilepticus in the hippocampus. In contrast no correlations between superoxide dismutase and catalase activities were observed. Our results suggest that GSH and catalase activity play an antioxidant role in the hippocampus during status epilepticus. Status epilepticus SE is a neurological .