tailieunhanh - Báo cáo khoa học: Interaction of HIV-1 with dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrinexpressing cells is influenced by gp120 envelope modifications associated with disease progression

Dendritic cells can enhance the replication of HIV-1 in CD4 + lymphocytes through the interaction of the gp120 envelope protein with such molecules as dendritic cell-specific intercellular adhesion molecule-3-grabbing noninte-grin. The variable loops of gp120 have previously been shown to modulate the interaction of HIV-1 with its principal receptor CD4 and its various coreceptors, namely CCR5 and CXCR4. | ễFEBS Journal Interaction of HIV-1 with dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-expressing cells is influenced by gp120 envelope modifications associated with disease progression Alexey A. Nabatov1 Thijs van Montfort1 Teunis B. H. Geijtenbeek2 Georgios Pollakis1 and William A. Paxton1 1 Department of Human Retrovirology Academic MedicalCenter University of Amsterdam the Netherlands 2 Department of Molecular Cell Biology and Immunology Vrije Universiteit MedicalCenter Amsterdam the Netherlands Keywords CD4 lymphocytes DC-SIGN gp120 HIV-1 Correspondence W. A. Paxton Department of Human Retrovirology Academic MedicalCenter University of Amsterdam Meibergdreef 15 1105 AZ Amsterdam the Netherlands Fax 31 20 691 6531 Tel 31 20 566 4739 E-mail Received 29 June 2006 revised 31 August 2006 accepted 6 September 2006 doi Dendritic cells can enhance the replication of HIV-1 in CD4 lymphocytes through the interaction of the gp120 envelope protein with such molecules as dendritic cell-specific intercellular adhesion molecule-3-grabbing noninte-grin. The variable loops of gp120 have previously been shown to modulate the interaction of HIV-1 with its principal receptor CD4 and its various coreceptors namely CCR5 and CXCR4. Here we utilized a panel of molecular cloned viruses to identify whether gp120 modifications can influence the virus interaction with immature dendritic cells or a cell line expressing dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin Raji-DC-SIGN . The viruses encompass the R5 R5X4 and X4 phenotypes and are based upon V1V2 and V3 sequences from a patient with disease progression. We found that dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin enhancement of virus replication can be modulated by the V1V2 length the overall V3 charge and N-linked glycosylation patterns similar results were observed with immature .