tailieunhanh - Báo cáo khoa học: Synthesis of b-mannosides using the transglycosylation activity of endo-b-mannosidase from Lilium longiflorum

Endo-b-mannosidase is an endoglycosidase that hydrolyzes only the Manb1-4GlcNAc linkage of the core region of N-linked sugar chains. Recently, endo-b-mannosidase was purified to homogeneity from Lilium longiflorum (Lily) flowers, its corresponding gene was cloned and import-ant catalytic amino acid residues were identified [Ishimizu T., Sasaki A., Okutani S., Maeda M., Yamagishi M. & Hase S. (2004)J. Biol. Chem. 279, 38555–38562]. | iFEBS Journal Synthesis of b-mannosides using the transglycosylation activity of endo-b-mannosidase from Lilium longiflorum Akiko Sasaki1 Takeshi Ishimizu1 Rudolf Geyer2 and Sumihiro Hase1 1 Department of Chemistry Graduate Schoolof Science Osaka University Japan 2 Institute of Biochemistry Faculty of Medicine University of Giessen Germany Keywords b-mannoside endo-b-mannosidase enzymatic synthesis N-glycan transglycosylation Correspondence Sumihiro Hase Department of Chemistry Graduate School of Science Osaka University 1-1 Machikaneyamacho Toyonaka Osaka 560-0043 Japan Fax 81 6 6850 5383 Tel 81 6 6850 5380 E-mail suhase@ Note The structures and abbreviations for the sugar chains are listed in Table 1 Received 8 December 2004 revised 23 January 2005 accepted 31 January 2005 doi Endo-b-mannosidase is an endoglycosidase that hydrolyzes only the Manpi-4GlcNAc linkage of the core region of N-linked sugar chains. Recently endo-b-mannosidase was purified to homogeneity from Lilium longiflorum Lily flowers its corresponding gene was cloned and important catalytic amino acid residues were identified Ishimizu T. Sasaki A. Okutani S. Maeda M. Yamagishi M. Hase S. 2004 J. Biol. Chem. 279 38555-38562 . In the presence of Manb1-4GlcNAcb1-4GlcNAc-pep-tides as a donor substrate and p-nitrophenyl b-N-acetylglucosaminide as an acceptor substrate the enzyme transferred mannose to the acceptor substrate by a pi-4-linkage regio-specifically and stereo-specifically to give Manpi-4GlcNAcpi-pNP as a transfer product. Further studies indicated that not only p-nitrophenyl b-N-acetylglucosaminide but also p-nitrophenyl b-glucoside and p-nitrophenyl b-mannoside worked as acceptor substrates however p-nitrophenyl b-N-acetylgalactosaminide did not work indicating that the configuration of the hydroxyl group at the C4 position of an acceptor is important. Besides mannose oligomannoses were also transferred. In the presence of Man .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• tumor cells
• medical knowledge
• chemical reaction
• Elements of Chemical Reaction Engineering
• Chemical Reaction Engineering
• Hóa học và ứng dụng
• Kỹ thuật hóa học
• Phản ứng hóa học
• Kỹ thuật phản ứng hóa học
• The chemical vapor deposition condition
• The electrochemically catalytic efficiency
• Hydrogen evolution reaction in MoS2 nanoparticles
• Hydrogen evolution reaction
• Metal organic chemical vapor deposition
• Reaction Kinetics
• Heterogeneous Catalysis
• Elementary Reactions
• Microkinetic Analysis
• Nonisothermal Reactors
• Catalytic Reactions
• Quantum chemical modeling mechanism
• Semiempirical method RM1
• Quantum chemical study
• Half chair shape
• Diels Alder reaction
• Chemical reaction engirneering
• Hoá phân tích
• Giải pháp phản ứng hóa học
• Radiation on MHD casson fluid flow
• Chemical reaction effects on MHD casson fluid flow
• Porous medium with suction injection
• The occurrence of suction/injection
• The central PDEs
• Radiation of MHD visco elastic fluid flow over
• Chemical reaction on heat of MHD visco elastic
• Mass transfer of MHD visco elastic fluid flow
• Exponentially stretching sheet through porous medium
• Radiation on the boundary layer MHD casson fluid
• Chemical reaction effects on the boundary layer MHD casson fluid
• Vertical plate embedded in the porous medium
• Casson fluid flow free convection boundary layer fl
• Extreme learning machine
• Artificial chemical reaction optimization algorithm
• Single hidden layer feed forward neural network
• Learning algorithm
• Chemical reaction kinetic
• Adsorption kinetics
• Pseudo first order model
• Pseudo second order model
• General kinetic model
• Dried Probiotics
• Industrial Processes
• Membrane Separation
• Wastewater
• Maillard Reaction
• Pros and Cons
• Chemical kinetics
• Reactant concentration
• Reaction rates and stoichiometry
• Action of catalysts
• Surface area
• Chemical kinetics
• Introduction to general
• Chemical bonds
• Chemical reactions
• Reaction rates
• Chemical equilibrium
• Nuclear chemistry
• Nuclear engineering and technology
• Correlation between rare earth elements
• The chemical interactions of HT9 cladding
• FueleCladding Chemical Interaction
• Higher reaction thickness
• Summary of Chemical PhD Thesis
• Theoretical and Physical Chemistry
• CO hydrogenation reaction
• Ni Cu bimetallic catalyst system
• Density functional method
• Chemical method
• Lecture General Chemistry 1
• Bài giảng Hóa học đại cương 1
• Balance chemical equations
• Calculate the percent yield of a reaction
• Chemical formulas
• Chemical equations
• organic chemistry
• hóa hữu cơ
• công nghệ hóa học
• Chemical Engineering
• common reaction mechanisms
• Chromatography in the petroleum industry
• oxidation
• BMC Bioinformatics
• Flux balance analysis
• Linear programming
• Design of metabolic network
• Constraint based model
• Growth rate
• Production rate
• Smaller reaction network
• Luận án Tiến sĩ
• Physical chemistry
• Fabbricated electrocatalysts
• Oxygen evolution reaction
• Proton exchange membrane
• breast cancer
• medicine
• cytotoxicity
• Atomic nucleus
• electrons
• Chemical compounds
• Electrochemistry