tailieunhanh - Báo cáo khoa học: SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc

During adipocyte differentiation, there is an underlying complex series of gene expressions. We have previously isolated many genes whose expres-sion levels are quickly elevated by the addition of inducers to mouse 3T3-L1 preadipocyte cells. Here we report the isolation and characterization of SLC39A14, a member of the LZT proteins, one of the subfamilies of ZIP transporters. | iFEBS Journal SLC39A14 a LZT protein is induced in adipogenesis and transports zinc Kei Tominaga1 2 Takeshi Kagata1 Yoshikazu Johmura1 Tomoaki Hishida1 Makoto Nishizuka1 and Masayoshi Imagawa1 1 Department of Molecular Biology Graduate Schoolof PharmaceuticalSciences Nagoya City University Aichi Japan 2 Research Division Nissui PharmaceuticalCo. Ltd Hokunanmoro Yuki Ibaraki Japan Keywords 3T3-L1 cells adipocyte differentiation LIV subfamily of ZIP transporters SLC39A14 Zrt Irt-like protein Correspondence M. Imagawa Department of Molecular Biology Graduate Schoolof Pharmaceutical Sciences Nagoya City University 3-1 Tanabe-dori Mizuho-ku Nagoya Aichi 467-8603 Japan Tel Fax 81 52 836 3455 E-mail imagawa@ Received 9 October 2004 revised 14 December 2004 accepted 24 January 2005 During adipocyte differentiation there is an underlying complex series of gene expressions. We have previously isolated many genes whose expression levels are quickly elevated by the addition of inducers to mouse 3T3-L1 preadipocyte cells. Here we report the isolation and characterization of SLC39A14 a member of the LZT proteins one of the subfamilies of ZIP transporters. The expression of the SLC39A14 gene was strongly and rapidly induced at the early stages of differentiation. Moreover it was highly restricted to the potential differentiation state of 3T3-L1 cells and the expression level was quite low in the nonadipogenic NIH-3T3 cells indicating a dominant expression in adipocyte differentiation. The zinc uptake assay revealed that SLC39A14 functions as a zinc transporter. Taken together these results suggest that SLC39A14 plays a role as a zinc transporter during the early stages of adipogenesis. doi Obesity is a major health problem in industrialised societies. It is related to the development of type 2 diabetes mellitus hypertension and arteriosclerosis 1 . Obesity often results in these kinds of life style-related diseases as the .