tailieunhanh - Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells

Although group IIA secretory phospholipase A2 (sPLA2-IIA) is known to be abundantly present in the joints of patients with rheumatoid arthritis (RA), expression of other sPLA2 s in this disease has remained unknown. In this study, we examined the expression and localization of six sPLA2s (groups IIA, IID, IIE, IIF, V and X) in human RA. Immunohistochemis-try of RA sections revealed that sPLA2 -IIA was generally located in syn-ovial lining and sublining cells and cartilage chondrocytes, sPLA2-IID in lymph follicles and capillary endothelium, sPLA2-IIE in vascular smooth muscle cells, and sPLA2-V in interstitial fibroblasts. . | ềFEBS Journal Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells Seiko Masuda1 Makoto Murakami1 Kazuo Komiyama2 Motoko Ishihara3 Yukio Ishikawa3 Toshiharu Ishii3 and Ichiro Kudo1 1 Department of Health Chemistry Schoolof PharmaceuticalSciences Showa University Tokyo Japan 2 Department of Pathology Division of Immunology and Patho-Biology at DentalResearch Center Nihon University Schoolof Dentistry Tokyo Japan 3 Department of Pathology Toho University Schoolof Medicine Tokyo Japan Keywords immunohistochemistry phospholipase A2 prostaglandin rheumatoid arthritis synovial cell Correspondence M. Murakami Department of Health Chemistry School of Pharmaceutical Sciences Showa University 1-5-8 Hatanodai Shinagawa-ku Tokyo 142-8555 Japan Fax 81 3 37848245 Tel 81 3 37848197 E-mail mako@ Received 8 May 2004 revised 26 October 2004 accepted 17 November 2004 doi Although group IIA secretory phospholipase A2 sPLA2-IIA is known to be abundantly present in the joints of patients with rheumatoid arthritis RA expression of other sPLA2s in this disease has remained unknown. In this study we examined the expression and localization of six sPLA2s groups IIA IID IIE IIF V and X in human RA. Immunohistochemistry of RA sections revealed that sPLA2-IIA was generally located in synovial lining and sublining cells and cartilage chondrocytes sPLA2-IID in lymph follicles and capillary endothelium sPLA2-IIE in vascular smooth muscle cells and sPLA2-V in interstitial fibroblasts. Expression levels of these group II subfamily sPLA2s appeared to be higher in severe RA than in inactive RA. sPLA2-X was detected in synovial lining cells and interstitial fibers in both active and inactive RA sections. Expression of sPLA2-IIF was partially positive yet its correlation with disease states was unclear. Expression of sPLA2 transcripts was also evident in .