tailieunhanh - Báo cáo khoa học: Human enhancer of rudimentary is a molecular partner of PDIP46/SKAR, a protein interacting with DNA polymerase d and S6K1 and regulating cell growth

Enhancer of rudimentary (ER) is a small protein that has a unique amino acid sequence and structure. Its highly conserved gene has been found in all eukaryotic kingdoms with the exception of fungi. ER was proposed to be involved in the metabolism of pyrimidines and was reported to act as a transcriptional repressor in a cell type-specific manner. | ễFEBS Journal Human enhancer of rudimentary is a molecular partner of PDIP46 SKAR a protein interacting with DNA polymerase Ô and S6K1 and regulating cell growth Amelia Smyk1 Magdalena Szuminska1 Katarzyna A. Uniewicz1 Lee M. Graves2 and Piotr Kozlowski1 1 Institute of Biochemistry Warsaw University Warsaw Poland 2 Department of Pharmacology University of North Carolina at Chapel Hill NC USA Keywords ER POLDIP3 pyrimidine RNA recognition motif yeast two-hybrid system Correspondence P. Kozlowski Institute of Biochemistry Warsaw University Miecznikowa 1 02-096 Warsaw Poland Fax 48 22 5543116 Tel 48 22 5543108 E-mail pkozlowski@ Databases The nucleotide sequence reported in this paper has been submitted to the DDBJ EMBL GenBank databases with the accession number DQ887818. The DDBJ EMBL GenBankaccession numbers U66871 ER AL160111 and AL160112 POLDIP3 . The UniProtKB SwissProt accession numbers P84090 ER P05990 CAD Q91901 DCoH PCD O00267 SPT5 Q9Y5B0 FCP1 Q9BY77 PDIP46 SKAR and P23443 S6K1 . Received 25 April 2006 revised 18 August 2006 accepted 18 August 2006 Enhancer of rudimentary ER is a small protein that has a unique amino acid sequence and structure. Its highly conserved gene has been found in all eukaryotic kingdoms with the exception of fungi. ER was proposed to be involved in the metabolism of pyrimidines and was reported to act as a transcriptional repressor in a cell type-specific manner. To further elucidate ER functions we performed the yeast two-hybrid screen of the human lung cDNA library for clones encoding proteins interacting with the human ER protein. The screen yielded polymerase Ỗ interacting protein 46 or S6K1 Aly REF-like target PDIP46 SKAR . a protein possessing one RNA recognition motif RRM and being a protein partner of both the p50 subunit of DNA polymerase Ỗ and p70 ribosomal protein S6 kinase 1 S6K1 . This interaction was further confirmed in vitro by the glutathione S-trans-ferase-ER pull-down of a protein of 46 kDa from a .