tailieunhanh - Báo cáo khoa học: Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins

Although the type-2 ribosome-inactivatingproteins (SNA-I, SNA-V, SNLRP) from elderberry (Sambucus nigraL.) are all devoid of rRNAN-glycosylase activity towards plant ribosomes, some of them clearly show polynucleotide– adenosine glycosylase activity towards tobaccomosaic virus RNA. This particular substrate specificity was exploited to further unravel the mechanism underlying the in planta antiviral activity of ribosome-inactivating proteins. Trans-genic tobacco (Nicotiana tabacumL. cv SamsunNN) plants expressing the elderberry ribosome-inactivating proteins were generated and challengedwith tobaccomosaic virus in order to analyze their antiviral properties. . | Eur. J. Biochem. 271 1508-1515 2004 FEBS 2004 doi Analysis of the in planta antiviral activity of elderberry ribosome-inactivating proteins Frank Vandenbussche1 Stijn Desmyter1 Marialibera Ciani2 Paul Proost3 Willy J. Peumans1 and Els J. M. Van Damme4 1 Laboratory for Phytopathology and Plant Protection Katholieke Universiteit Leuven Belgium 2Dipartimento di Patologia sperimentale Universita di Bologna Italy 3Rega Institute Department of Microbiology and Immunology Katholieke Universiteit Leuven Belgium 4Department of Molecular Biotechnology Gent University Belgium Although the type-2 ribosome-inactivating proteins SNA-I SNA-V SNLRP from elderberry Sambucus nigra L. are all devoid of rRNA N-glycosylase activity towards plant ribosomes some of them clearly show polynucleotideadenosine glycosylase activity towards tobacco mosaic virus RNA. This particular substrate specificity was exploited to further unravel the mechanism underlying the in planta antiviral activity of ribosome-inactivating proteins. Transgenic tobacco Nicotiana tabacum L. cv Samsun NN plants expressing the elderberry ribosome-inactivating proteins were generated and challenged with tobacco mosaic virus in order to analyze their antiviral properties. Although some transgenic plants clearly showed antiviral activity no clear correlation was observed between in planta antiviral activity of transgenic tobacco lines expressing the different ribosome inactivating proteins and the in vitro polynucleotideadenosine glycosylase activity of the respective proteins towards tobacco mosaic virus genomic RNA. However our results suggest that the in planta antiviral activity of some ribosome-inactivating proteins may rely on a direct mechanism on the virus. In addition it is evident that the working mechanism proposed for pokeweed antiviral protein cannot be extrapolated to elderberry ribosome-inactivating proteins because the expression of SNA-V is not accompanied by induction of .

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